The effect of citrate and sulfate of different metals on carbohydrates composition of medicinal mushroom Trametes versicolor (Polyporales, Polyporaceae)
Abstract
The aim of our research was to study the influence of citrate and sulfate of copper, manganese and zinc on the carbohydrates composition of biomass of the medicinal mushroom Trametes versicolor (L.) Lloyd. cultivated in a liquid medium. The studied strain of Trametes versicolor 353 was obtained from the Culture Collection of Mushrooms (IBK) from the M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. Citrates of copper, manganese and zinc were obtained from the Institute of Nanobiotechnologies and Resource Conservation of Ukraine, Kyiv. In this study we used glucose-peptone-yeast extract medium (GPY) with the following composition of (g/L): glucose – 25, peptone – 3, yeast extract – 3, K2HPO4 – 1, KH2PO4 – 1, MgSO4 ∙ 7H2O – 0.25, distilled water – 1,000 ml; pH 6,5 (control medium). Cu2+ (sulfate form or citrate form) were added to control medium in concentration 4 mg/L, Zn2+ and Mn2+ (sulfate form or citrate form) were added to control medium in concentration 1 mg/L. Mycelium was grown in a submerged culture on a rotary shaker (120 rpm) at 26 °C in 250 ml Erlenmeyer flasks, containing 50 ml of liquid medium. The biomass was harvested after 9 days of cultivation in the liquid medium. Monosaccharides and sugar alcohols was analyzed by high-performance liquid chromatography Agilent 1200 (Agilent technologies, USA). The results of our research demonstrated that the fraction of total carbohydrates from T. versicolor 353, which were cultivated on the control medium, consists of glucose (91.5%), galactose (1.9%), mannose (5.4%), fucose (0.6%) and xylose (0.7%). The adding of sulfate of zinc or copper to the liquid medium didn’t change the content of monosaccharides of T. versicolor 353 biomass relative to the control medium. At the same time, in the medium with citrate of zinc the amount of glucose increased and the amount of mannose decreased relative to the control medium. Xylose and fucose were not detected in this case. In mycelium of T. versicolor 353 that was cultivated in the medium with manganese sulfate or manganese citrate we detected a small amount of ribose. But the content of other monosaccharides didn’t change in the medium with sulfate of manganese. At the same time, the addition of citrate of manganese to the medium raised the amount of mannose and reduced the amount of glucose in the biomass of T. versicolor 353 relative to the control medium. Also copper citrate reduced the content of glucose in mycelium of T. versicolor 353 relative to the control medium. But in this case we detected mannitol and sorbitol in the biomass. This data indicates that synthesis of sugur alcohols in T. versicolor 353 depend on copper ions in bioactive forms of citrate.References
Al-Maali, G.A., 2015. The influence of metal citrates obtained by aquananotechnology on growth of the strains of medical macromycetes Ganoderma lucidum 1900 and Trametes versicolor 353. Ukr. Bot. J. 72(4), 393–397.
Antonenko, L.A., 2013. Biotekhnolohiia otrymmannia biomasy vyshchykh bazydialnykh hrybiv rodu Coriolus [Biotechnology of biomass higher basidiomycetes of the genus Coriolus]. NUHT, Kyiv (in Ukrainian).
Banci, L., 2013. Metallomics and the cell. Springer, Dordrecht.
Bisko, N.A., Babickaya, V.G., Buchalo, A.S., Krupoderova, T.A., Lomberg, M.L., Mikhajlova, O.B., Puchkova, N.A., Solomko, E.F., Shcherba, V.V., 2012. Biologicheskie svojstva lekarstvennyh makromicetov v kul’ture [Biological properties of the medicinal macromycetes in the culture]. Laposhshenko LTD, Kyiv (in Russian).
Broadley, M.R., White, P.J., Hammond, J.P., Zelko, I., Lux, A., 2007. Zinc in plants. New Phytol.t 173(4), 677–702. >> doi:10.1111/j.1469-8137.2007.01996.x
Buchalo, A.S., Mytropolska, N.Y., Mykchaylova, O.B., 2011. Catalogue of the culture collection of mushrooms IBK. Alterpress, Kiev.
Cai, X., Pi, Y., Zhou, X., Tian, L., Qiao, S., Lin, J., 2010. Hepatoma cell growth inhibition by inducing apoptosis with polysaccharide isolated from Turkey tail medicinal mushroom, Trametes versicolor (L.: Fr.) Lloyd (Aphyllophoromycetideae). Int. J. Med. Mushr. 12(3), 257–263.
Hobbs, C., 2004. Medicinal value of Turkey tail fungus Trametes versicolor (L.:Fr.) Pilát (Aphyllophoromycetideae). Int. J. Med. Mushr. 6(3), 195–218.
Jannings, D.H., 1984. Polyol metabolism in fungi. Adv. Microbiol. Phisiol. 25, 149–193. >> doi:10.1016/S0065-2911(08)60292-1
Kamzolkina, O.V., Dunaevskaya, J.A., 2015. Fungal cell biology [Biologiya gribnoj kletki]. KMK, Moscow (in Russin).
Kozarski, M., Klaus, A., Nikšić, M., Vrvić, M.M., Todorović, N., Jakovljević, D., Van Griensven, L.J., 2012. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. J. Food Compos. Anal. 26(1), 144–153. >> doi:10.1016/j.jfca.2012.02.004
Kroneck, P.M.H., Sosa Torres, M.E. (eds.), 2015. Sustaining life on planet Earth: Metalloenzymes mastering dioxygen and other chewy gases. Springer International Publishing, Switzerland. >> doi:10.1007/978-3-319-12415-5
Kuan, Y.C., Wu, Y.J., Hung, C.L., Sheu, F., 2013. Trametes versicolor protein YZP activates regulatory B lymphocytes – gene identification through de novo assembly and function analysis in a murine acute colitis model. PloS One, 8(9), e72422. >> doi:10.1371/journal.pone.0072422
Law, N., Caudle, M., Pecoraro, V., 1998. Manganese redox enzymes and model systems: Properties, structures, and reactivity. Adv. Inorg. Chem. 46, 305–440. >> doi:10.1016/S0898-8838(08)60152-X
Lee, J.K., Oh, D.K., Song, H.Y., Kim, I.W., 2007. Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae. Biotechnol. Lett. 19, 291–294. >> doi:10.1007/s10529-006-9230-4
Maehara, Y., Tsujitani, S., Saeki, H., Oki, E., Yoshinaga, K., Emi, Y., Baba, H., 2012. Biological mechanism and clinical effect of protein-bound polysaccharide K (Krestin®): Review of development and future perspectives. Surgery Today 42(1), 8–28. >> doi:10.1007/s00595-011-0075-7
Montoya, S., Sanchez, O.J., Levin, L., 2013. Polysaccharide production by submerged and solid-state cultures from several medicinal higher Basidiomycetes. Int. J. Med. Mushr. 15(1), 71–79. >> doi:10.1615/IntJMedMushr.v15.i1.80
Patel, S., Goyal, A., 2012. Recent developments in mushrooms as anti-cancer therapeutics: A review. 3 Biotech. 2(1), 1–15. >> doi:10.1007/s13205-011-0036-2
Pharr, D.M., Stoop, J.M.H., Williamson, J.D., Feusi, M.E.S, Massel, M.O., Conkling, M.A., 1995. The dual role of mannitol as osmoprotectant and photoassimilate in celery. Hort Sci. 30, 1182–1188.
Ren, Y., Perepelov, A.V., Wang, H., Zhang, H., Knirel, Y.A., Wang, L., Chen, W., 2010. Biochemical characterization of GDP-L-fucose de novo synthesis pathway in fungus Mortierella alpina. Biochem. Bioph. Res. Co. 391(4), 1663–1669. doi:10.1016/j.bbrc.2009.12.116
Smilley, K.L., Cadmus, M.C., Liepins, P., 1967. Biosynthesis of D-mannitol from D-glucose by Aspergillus candidus. Biotechnol. Bioeng. 9, 365–374.
Song, H.S., Vieille, C., 2009. Recent advances in the biological production of mannitol. Appl. Microbiol. Biotechnol. 84, 55–62. >> doi:10.1007/s00253-009-2086-5
Standish, L.J., Wenner, C.A., Sweet, E.S., Bridge, C., Nelson, A., Martzen, M., Torkelson, C., 2008. Trametes versicolor mushroom immune therapy in breast cancer. J. Soc. Integr. Oncol. 6(3), 122–128.
Stoop, J.M., Mooibroek, H., 1998. Clonning and characterizasion of NADH-mannitol dehydrogenase cDNA from the button mushroom, Agaricus bisporus, and his expresion in response to NaCl stress. Appl. Environ. Microbiol. 64, 4689–4696.
Stoop, J.M.H., Williamson, J.D., Pharr, D.M., 1996. Mannitol metabolism in plants: Method for coping with stress. Trends Plant Sci. 1, 139–144. >> doi:10.1016/S1360-1385(96)80048-3
Valentine, B.P., Bainbridge, B.W., 1978. The relevance of a study of a temperature-sensitive ballooning mutant of Aspergillus nidulans defective in mannose metabolism to our understanding of mannose as a wall component and carbon/energy source. Microbiology 109(1), 155–168. >> doi:10.1099/00221287-109-1-155
Wang, F., Zhang, J., Hao, L., Jia, S., Ba, J., Niu, S., 2012. Optimization of submerged culture conditions for mycelial growth and extracellular polysaccharide production by Coriolus versiolor. J. Bioprocess Biotech. 2(4), 124–129. >> doi:10.4172/2155-9821.1000124
Wasser, S.P., 2014. Medicinal mushroom science: Current perspectives, advances, evidences, and challenges. Biomed. J. 37(6), 345–356. >> doi:10.4103/2319-4170.138318
Wasser, S.P., Weis, A.L., 1999. Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: A modern perspective. Crit. Rev. Immunol. 19(1), 65–96.
Xiao, J.H., Chen, D.X., Wan, W.H., Hu, X.J., Qi, Y., Liang, Z.Q., 2006. Enhanced simultaneous production of mycelia and intracellular polysaccharide in submerged cultivation of Cordyceps jiangxiensis using desirability functions. Process Biochem. 41(8), 1887–1893. >> doi:10.1016/j.procbio.2006.03.031
Zhang, H., Li, Q., He, P., Xu, C., 2015. Effect of carbon source on properties and antioxidant potential of exopolysaccharides produced by Trametes robiniophila (Higher Basidiomycetes). Int. J. Med. Mushrooms 17(2), 179–186. >> doi:10.1615/IntJMedMushrooms.v17.i2.90
Zhi-ling, C., 2009. Effect of some trace elements and vitamins on contents of polysaccharide and acid of Ganoderma lucidum. Journal of Anhui Agricultural Sciences 5, 078.
Zong, A., Cao, H., Wang, F., 2012. Anticancer polysaccharides from natural resources: A review of recent research. Carbohydr. Polym. 90(4), 1395–1410. >> doi:10.1016/j.carbpol.2012.07.026
Zou, X., 2005. Effects of Zn supplementation on the growth, amino acid composition, polysaccharide yields and anti-tumour activity of Agaricus brasiliensis. World J. Microb. Biot. 21(3), 261–264. >> doi:10.1007/s11274-004-2614-5
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