The influence of oxidative stress on the state of the antioxidant defense system in the organism of rats
Abstract
This article presents the results of research on the influence of oxidative stress on the intensity of the process of lipid peroxidation and the activity of the glutathione system of antioxidant defense in the organisms of rats. Intramuscular injection of 50% solution of tetrachloromethane at a dose of 0.25 ml per100 gof body weight to rats from the experimental group causes activation of the process of free radical lipid oxidation with excessive accumulation of intermediate anf final products of lipid peroxidation. The research results indicate that the development of oxidative stress leads to significant and probable acceleration of the formation and accumulation in the plasma of the rats, in all stages of the experiment, of lipid hydroperoxides and malonic dialdehyde. The highest level of hydroperoxides of lipids in the blood plasma of rats under oxidative stress was on the second day of the experiment, where it was 843 unE/ml, whereas in the control this index was 0.245 unE/ml. During the research into the content of malondialdehyde it was found that in the experimental group of animals it was 2.03 times higher than in the control group on the 5th day. On the 10th and 14th days of the experiment we observed a slight reduction in the levels of lipid hydroperoxides and malondialdehyde. The development of oxidative stress also leads to inhibition of the glutathione system of antioxidant defense in the rats’ organism. This shows the low activity of glutathione peroxidase and the low level of restored glutathione in the blood of the rats from the experimental group. On the 5th day of experiment the activity of glutathione peroxidase and restored glutathione level in the blood of the rats which were injected with carbon tetrachloride was at its lowest, compared with the control these indices decreased respectively by 53% and 51%. On the 10th and 14th days of the experiment the activity of glutathione peroxidase and restored glutathione level in the blood of the rats from the experimental group were slightly increased, but compared to the control they were still significantly lower. Significant disturbances were found in the oxidation-antioxidant balance of the animals under oxidative stress, which is characterized by the activation of the processes of free radical lipid oxidation with excessive accumulation of intermediate and final products and the inhibition of the antioxidant defense system.References
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