Dynamics of indices of aminotransferase activity in the blood serum of rats under conditions of oxidative stress and effect of liposomal medicinal product
Abstract
The article presents the results of testing the effect of a developed complex liposomal medicinal product on the dynamics of aminotransferase activity indices of the organisms of rats under conditions of designed oxidative stress induced by means of carbon tetrachloride application. It was demonstrated that intramuscular administration to rats from the tested groups of 50% tetrachloroethene at the dose of 0.25 ml to100 gof body weight causes antigen loading on the organism and increase in the cell membrane penetrability of hepatocytes and mitochondrial membranes. This was demonstrated by increase in activity of aminotransferase in the blood serum during the whole period of the experiment. On the second day of the test aspartate transaminase increased 2.5 times, alanine aminotransferase twice and the coefficient of AST/ALT by 30% in comparison with the control group. On the 14th day of the test the activity of these indices was higher compared to the indices in the control group, AST and ALT by 93% and 81% respectively, the coefficient index was higher and was equal to 2.89 ±0.05 incomparison to the index for the control (2.72 ± 0.04), that is 9% higher than the control indices. In order to normalize cell membrane penetrability of hepatocytes and mitochondrial membranes under conditions of oxidative stress, it is useful to apply a liposomal medicinal product containing butafosfan, interferon, cardus marianus and vitamins. The application of the liposomal medicinal product to rats under the experimental conditions of oxidative stress caused the normalization of enzyme activity of reamidation in the blood, namely normalization of AST and ALT activity in the blood serum. On the 14th day the indices of enzyme activity of aminotransferase in the blood of the tested rats fluctuated within the physiological values that demonstrate the normalization of cell membrane penetrability of hepatocytes and mitochondrial membranes under conditions of oxidative stress and the liposomal effect of the medicinal product.References
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