Comparison of gene expression of metallothioneins, ubiquitin and p53 in fibroblasts from lung and skin of rats of different age


  • Y. G. Kot V.N. Karazin Kharkiv National University
  • E. V. Kot V.N. Karazin Kharkiv National University
  • E. S. Morosova V.N. Karazin Kharkiv National University
  • E. E. Persky V.N. Karazin Kharkiv National University
  • M. A. Gritsenko V.N. Karazin Kharkiv National University
  • N. I. Bulankina V.N. Karazin Kharkiv National University
Keywords: culture of fibroblasts, ontogeny, metallothioneins, ubiquitin, p53

Abstract

We studied gene expression of five metallothioneins (MT 1-5), ubiquitin and protein p53 and their products in fibroblasts culture of the skin and lungs of white rats of different ages (2 weeks, 1, 3, and 24 months) and determined its (metallothionein 1-5 types, ubiquitin, p53) product quantity. All these proteins are protective ones, but perform their functions by using different mechanisms. Metallothionein bind, transport and excrete ions of bivalent metals, ubiquitin controls the cleavage of the defective and short-lived proteins in the proteasome, protein p53 controls apoptosis, thus ensuring the genome stability. The similarity of age dynamics of gene expression of ubiquitin and MT of cells of both sources has been shown – maximum at 3 months. Expression of p53 gene has a difference: both in the skin and lungs expression increases up to 24 months. Product quantity of p53 has a minimum in the skin at 3 months and remains constant; in the lungs, this value has a maximum at 1 month.

References

Ali-Bahar, M., 2004. Dermal fibroblasts from different layers of human skin are heterogeneous in expression of collagenase and types I and III procollagen mRNA. Wound Repair Regen. 12(2), 175–182. >> doi: 10.1111/j.1067-1927.2004.012110.x
Barder, N., Grune, T., 2006. Protein oxidation and proteolysis. Biol. Chem. 387(10-11), 1351–1355.
Blagosklonny, M.V., 2010. Calorie restriction: Decelerating mTOR-driven aging from cells to organisms (including humans). Cell Circle 9(4), 683–688. >> doi: 10.4161/cc.9.4.10766
Blagosklonny, M.V., Hall, M.N., 2009. Growth and aging: A common molecular mechanism. Aging 1(4), 357–362. >> doi: 10.18632/aging.100040
Chang, H.Y., Chi, J.-T., Dudoit, S., Bondre, C., van de Rijn, M., Botstein, D., Brown, P.O., 2002. Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc. Natl. Acad. Sci. USA 99(20), 12877–12882. >> doi: 10.1073/pnas.162488599
Chumakov, P.M., 2007. Belok p53 i ego universal'nye funkcii v mnogokletochnom organizme [The p53 protein and its universal function in a multicellular organism]. Uspehi Biologicheskoj Himii, 47, 3–52.
Davis, S.R., Cousins, R.J., 2000. Metallothionein expression in animals: Physiological perspective on function. J. Nutr. 130, 1085–1088.
Degterev, A., Yuan, J., 2008. Expansion and evolution of cell death programmes. Nat. Rev. Mol. Cell Biol. 9, 378–390. >> doi: 10.1038/nrm2393
Harrison, D.E., Strong, R., Sharp, Z.D., Nelson, J.F., Astle, C.M., Flurkey, K., Nadon, N.L., Wilkinson, J.E., Frenkel, K., Carter, C.S., Pahor, M., Javors, M.A., Fernandez, E., Miller, R.A., 2009. Rapamycin fed late in life extends lifespan in genetically heterogeneous mice. Nature 460, 392–395. >> doi: 10.1038/nature08221
Hoyt, M.A., Zich, J., Zhang, M., Govaerts, C., Coffino, P., 2006. Glycine-alanine repeats impair proper substrate unfolding by the proteasome. EMBO J. 25(8), 1720–1729. >> doi: 10.1038/sj.emboj.7601058
Jang, T., Catalgol, B., Grune, T., 2009. Age related differences in oxidative protein damage in young and senescent fibroblasts. Arch. Biochem. Biophys. 483, 127–135. >> doi: 10.1016/j.abb.2008.12.007
Johnson, S.C., Rabinovitch, P.S., Kaeberlein, M, 2013. mTOR is a key modulator of agening and age-related disease. Nature 493, 338–345.
Li, W., Ye, Y., 2008. Polyubiquitin chains: Functions, structures, and mechanisms. Cell. Mol. Life Sci. 65, 2397–2406. >> doi: 10.1007/s00018-008-8090-6
Liuzzi, J.P., Blanchard, R.K., Cousins, R.J., 2001. Differential regulation of zinc transporter 1, 2, and 4 mRNA expression by dietary zinc in rats. J. Nutr. 131, 46–52.
Muller, F.L., Lustgarten, M.S., Jang, Y., Richardson, A., Van Remmen, H., 2007. Trends in oxidative aging theories. Free Radical Bio. Med. 43, 477–503. >> doi: 10.1016/j.freeradbiomed.2007.03.034
Pyhteeva, E.G., 2009. Metallotioneiny: Biologicheskaja funkcija. Rol' metallotioneinov v transporte metallov v organizme [Metallothioneins: Biological function. Role of metallothioneins in the transport of metals in the body]. Aktual'nye Problemy Transportnoj Mediciny 4(18), 44–58.
Ran, Q., Liang, H., Ikeno, Y., Qi, W., Prolla, T.A., Roberts II, L.J., Wolf, N., VanRemmen, H., Richardson, A., 2007. Reduction in glutathion peroxidase 4 increases life span through increased sensitivity to apoptosis. J. Gerontol. Biol. Sci. Med. Sci. 62(9), 932–942.
Rinn, J.L., Bondre, C., Gladstone, H.B., Brown, P.O., Chang, H.Y., 2006. Anatomic demarcation by positional variation in fibroblast gene expression programs. PLoS Genet. 2(7), 119. >> doi: 10.1371/journal.pgen.0020119
Rodríguez-Rodero, S., Fernandez Morera, J.L., Fernandez, A.F., Menendez Torre, E., Fraga, M.F, 2010. Epigenetic regulation of aging. Discovery Medicine 10(52), 225–233.
Salminen, A., Hyttinen, J.M.T., Kauppinen, A., Kaarniranta, K., 2012. Context-dependent regulation of autophagy by IKK-NF-kB signaling: Impact on the aging process. Int. J. Cell Biol. 849541.
Sharp, Z.D., 2011. Aging and TOR: Interwoven in the fabric of life. Cell. Mol. Life Sci. 68(4), 587–597. >> doi: 10.1007/s00018-010-0542-0
Thirumoorthy, N., Sunder, A.S., Manisenthil Kumar, K.T., Senthil Kumar, M., Ganesh, G.N.K., Chatterjee, M., 2011. A review of metallothionein isoforms and their role in pathophysiology. World J. Surg. Oncol. 9, 54. >> doi: 10.1186/1477-7819-9-54
Vousden, K.H., Lane, D.P., 2007. p53 in health and disease. Nat. Rev. Mol. Cell Biol. 8(8), 275–283. >> doi: 10.1038/nrm2147
Published
2015-09-24
How to Cite
Kot, Y., Kot, E., Morosova, E., Persky, E., Gritsenko, M., & Bulankina, N. (2015). Comparison of gene expression of metallothioneins, ubiquitin and p53 in fibroblasts from lung and skin of rats of different age. Regulatory Mechanisms in Biosystems, 6(2), 161-164. https://doi.org/https://doi.org/10.15421/021529

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