Toxicological assessment of a bioactive adaptogenic drug for use in rats and pigs
Abstract
Glycine and succinic acid elicit a potent stress response. They are not just anti-stress agents; they form a highly effective bioregulatory complex that quickly mobilizes animals' adaptive reserves and significantly reduces the impact of environmental stressors. Their synergistic action achieves a new level of effectiveness when combined with bioactive substances that enhance their physiological effects. The primary focus in this context is the development of a multicomponent adaptogenic drug based on glycine, succinic acid, and a biologically active medium 199, and its toxicological evaluation in rats and pigs. It has been esta b lished that a single intragastric administration of the bioactive adaptogenic preparation to male rats at doses of 1000 and 3000 mg/kg body weight did not result in any adverse effects on their clinical condition. Administering a dose of 5000 mg/kg of body weight was accompanied by a transient decrease in motor activity during the first two days after administration. When 9000 and 10000 mg/kg doses were applied to the skin of female rats, mild erythema was observed and disappeared within two hours. Field testing of the therapeutic dose (1.5 mL) demonstrated the drug's high efficacy in rapidly restoring behavioral responses, reducing stress levels, and normalizing physiological parameters in boars after castration. A pathological examination revealed no significant changes in the internal organs except for a probable increase in spleen mass index in female rat s that received 2.0 mL on the tenth day, likely due to increased sensitivity to the drug. Rats that received a 1.5 mL dose of the drug demonstrated i n creased hemoglobin, red blood cell, and total protein levels, as well as lower glucose levels. This is a characteristic manifestation of better adaptation to stressful conditions. Overall, the drug is classified as practically non-toxic in terms of toxicity and as low-toxicity in terms of hazard level. This study will further evaluate adaptogenic anti-stress drugs in mitigating the negative effects of technological stressors on pigs at various stages of the rearing cycle.References
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