Tumorigenicity assay of adrenal medulla derived cell culture
Abstract
Neurodegenerative disorders involve a continual and permanent deterioration of specific neuronal populations, leading to severe and debilitating neurological impairments. Despite the development of various therapeutic strategies, current treatments largely provide only symptomatic relief and are unable to halt or reverse disease progression. In this context, cell-based ther a pies using stem or progenitor cells offer a promising alternative. Neural crest-derived cells (NCDCs) represent a multipotent cell population that originates during embryogenesis and can give rise to diverse cell types. Importantly, these cells or their derivatives persist in certain adult tissues such as skin, dental pulp, and the adrenal medulla, making them accessible for bi o medical applications. Their high plasticity and availability from multiple sources make NCDCs a valuable candidate for reg e nerative medicine, including treatment of neurodegenerative disorders. However, one of the key safety requirements for cell u lar therapeutics is the evaluation of tumorigenic potential. In this study, we assessed the tumorigenicity of a cell culture derived from the adrenal medulla of neonatal pigs after subcutaneous transplantation into Balb/c mice. Immunodeficiency was induced by intraperitoneal administration of cyclosporine A. The Neuro2a neuroblastoma cell line was used as a tumorigenic positive control. Tumors developed in the groups that received Neuro2a cells (in 100% of the animals under immunosuppression and in 40% of the animals without it). No neoplasms were detected in the groups that received AGCC. Histological analysis co n firmed the presence of malignant tumors only in the groups injected with Neuro2a cells. The obtained results indicate the a b sence of tumorigenic potential in AGCC and justify the feasibility of their further preclinical studies.References
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