Analysis of the calving-to-conception interval variation and it relation to milk production traits of dairy cows
Abstract
Intensive selection for milk production has resulted in unfavorable genetic correlations with fertility, contributing to longer calving intervals, increased insemination rates, higher culling levels, and rising costs of reproductive management. This study e x amined the effects of genetic and environmental factors on the calving-to-conception interval and its relationship with milk produ c tivity traits in Holstein cows. The analysis was based on data from cows that calved at PJSC ‘Plemzavod Stepnoy’ in Zaporizhzhia region , Ukraine , between 2014 and 2017. For each animal, 305-d milk yield, milk fat percentage, and the calving-to-conception interval were recorded and analyzed using the General Linear Model. The results showed that parity, year and season of calving significantly influenced the calving-to-conception interval, whereas the effect of sire was not significant. Younger cows generally conceived sooner after calving than older cows. Over the study period, there was a clear tendency toward shorter calving-to-conception intervals, suggesting improvement in reproductive performance. Cows calving in summer and autumn also had shorter intervals than those calving in winter or spring. The joint analysis of parity and the calving-to-conception interval indicated that reproductive efficiency was closely related to milk yield and composition. Cows with moderate calving-to-conception intervals (91–120 d) produced more milk of slightly higher quality than those with shorter or longer intervals. Overall, the findings highlight the importance of managing reproductive cycles to optimize both fertility and milk productivity in Holstein herds under the produ c tion conditions of southern Ukraine.References
Alemu, T. W., Santschi, D. E., Cue, R. I., & Duggavathi, R. (2023). Reproductive performance of lactating dairy cows with elevated milk β-hydroxybutyrate levels during first 6 weeks of lactation. Journal of Dairy Science, 106(7), 5165–5181.
Ali, M. A. E., & Sanad, S. S. (2021). Factors affecting abortion and stillbirth and their effects on the subsequent reproductive performance of cows under Egyptian conditions. Egyptian Journal of Applied Science, 36(5–6), 79–94.
Almeida, R. D., Doska, M. C., Horst, J. A., Valloto, A. A., Santos, G. T. D., & Lima, L. S. D. (2021). Associations of days open with milk urea nitrogen and other herd traits in dairy cows. Revista Brasileira de Zootecnia, 50, e20200081.
Al-Murrani, W. (2024). General, biological and biomedical statistics. Cambridge Scholars Publishing, Newcastle.
Amma, Z., Reiczigel, J., Fébel, H., & Solti, L. (2024). Relationship between milk yield and reproductive parameters on three Hungarian dairy farms. Veterinary Sciences, 11(5), 218.
Arens, S. C., Sharpe, K. T., Schutz, M. M., Hardie, L. C., Dechow, C. C., & Heins, B. J. (2023). Relationships of beta-casein genetics with production, fertility, and survival of purebred organic Holstein dairy cows. JDS Communications, 4(6), 458–463.
Barzehkar, R., Emamjomeh Kashan, N., Asadi Fozi, M., & Chamani, M. (2023). A study on the association of days open with the genetic ranking of Iranian Holstein bulls for the trait of milk yield. Journal of Agricultural Science and Technology, 25(2), 331–339.
Beribe, M. J., Carignano, H. A., Poli, M. A., & Lopez-Villalobos, N. (2024). Effects of breed and genomic inbreeding on milk, fat and protein lactation yields and fertility traits in pasture-based dairy cows in Argentina. The Journal of Agricultural Science, 162(2), 181–189.
Boujenane, I., & Draga, B. (2021). Non-genetic factors affecting reproductive performance of Holstein dairy cows. Livestock Research for Rural Development, 33(1), 10.
Cañizares-Martínez, M. A., Parra-Bracamonte, G. M., Segura-Correa, J. C., & Magaña-Monforte, J. G. (2021). Effect of leptin, pituitary transcription factor and luteinizing hormone receptor genes polymorphisms on reproductive traits and milk yield in Holstein cattle. Brazilian Archives of Biology and Technology, 64, e21190643.
Chafai, N., Badaoui, B., & Rekaya, R. (2024). Genetic parameters of milk yield and fertility traits in Moroccan Holsteins. Frontiers in Animal Science, 5, 1446989.
Chuck, G. M., Mansell, P. D., Stevenson, M. A., & Izzo, M. M. (2024). Early life events associated with first lactation reproductive performance in southwest Victorian pasture-based dairy herds. Australian Veterinary Journal, 102(3), 51–59.
Constantin, G., & Mihaela, R. (2023). Causes and effects of stillbirths on days open and cow herd survival in Holstein Friesian cows. Open Journal of Veterinary Medicine, 13(3), 23–32.
Didanna, H. L., & Asmirew, L. (2025). A study on the lactation and reproductive performances of purebred Jersey stock: Implications for introducing European dairy breeds in the tropical environment. Journal of the Indonesian Tropical Animal Agriculture, 50(2), 68–81.
Dominguez-Castaño, P., Vargas de Oliveira, M. H., El Faro, L., & de Vasconcelos Silva, J. A. I. (2020). Relationship between reproductive and productive traits in Holstein cattle using multivariate analysis. Reproduction in Domestic Animals, 55(7), 770–776.
Durán-Alvarez, C., García-Ruiz, A., Alonso Morales, R. A., Eguiarte, L. E., & Ruiz-López, F. D. J. (2023). Genetic parameters, correlations and trends of reproductive traits in Holstein cattle from Mexico. Revista Mexicana de Ciencias Pecuarias, 14(3), 539–555.
Easa, A., Rashad, A. M., & Saleh, A. A. (2024). Evaluation of some economic traits in Holstein dairy cattle under Egyptian conditions. Journal of Advanced Veterinary Research, 14(1), 198–203.
El-Bakly, M. K., Mostafa, I. A., & Mahboub, H. D. (2023). Evaluation of reproductive performance and disorders in Holstein-Friesian and its crossbreds with Montbeliarde under Egyptian conditions. Alexandria Journal of Veterinary Sciences, 77(1), 115–122.
El-Komy, S. M., & Rashad, A. M. (2021). Genetic evaluation for productive and reproductive traits in Holstein Friesian cows under subtropical condition. Indian Journal of Animal Sciences, 91(11), 931–934.
Elmaghraby, M. A., Ngounndji, A., Fathala, M. M., & Sahwan, F. M. (2025). Cow, season and disease risk factors associated with subfertility in Holstein cows. Egyptian Journal of Veterinary Sciences, 56(5), 863–874.
El-Sherief, A. A., El-Komy, S. M., Rashad, A., & El-Hedainy, D. K. (2022). Reproductive performance of lactating Holstein cows as influenced by season of calving and parity under subtropical conditions. Journal of Advanced Veterinary Research, 12(1), 11–17.
Estrada-León, R. J., Valladares-Rodas, M. A., Vázquez, A. C. S., Monforte, J. G. M., Correa, J. C. S., & Parra-Bracamonte, G. M. (2024). Genetic parameters for milk yield and reproductive traits in Honduran Holstein cattle. Tropical Animal Health and Production, 56(5), 175.
Fahim, N. H., Ibrahim, M. A. A. M., Amin, A. H., & Sadek, R. R. (2021). Milk production and reproductive performance of retained and culled cows in a large Holstein herd in Egypt. World's Veterinary Journal, (3), 474–483.
Fathoni, A., Boonkum, W., Chankitisakul, V., Buaban, S., & Duangjinda, M. (2024). Integrating genomic selection and a genome-wide association study to improve days open in Thai dairy Holstein cattle: A comprehensive genetic analysis. Animals, 15(1), 43.
Ghiasi, H., Piwczyński, D., Sitkowska, B., & González-Recio, O. (2021). New composite traits for joint improvement of milk and fertility trait in Holstein dairy cow. Animal Bioscience, 34(8), 1303.
Gritsienko, Y., Karatieievа, O., & Gill, M. (2024). Identification of some genetic markers as productive and reproductive traits in Ukrainian dairy cattle breeding. Online Journal of Animal and Feed Research, 14(2), 124–136.
Habib, A. A., Gouda, G. F., El-Sayed, M., & Shemeis, A. R. (2024). Partial restricted selection indexes to fix the length of days open in high – yielding dairy cows. International Journal of Veterinary Science, 13(6), 833–840.
Hajibemani, A., & Mirzaei, A. (2022). Determination of predictive factors for the clinical cure rate of endometritis in Holstein dairy cows. Journal of the Hellenic Veterinary Medical Society, 73(4), 4971–4978.
Hu, H. H., Li, F., Mu, T., Han, L. Y., Feng, X. F., Ma, Y. F., Jiang, Y., Xue, X. S., Du, B. Q., Li, R. R., & Ma, Y. (2023). Genetic analysis of longevity and their associations with fertility traits in Holstein cattle. Animal, 17(6), 100851.
Jagusiak, W., Ptak, E., Otwinowska-Mindur, A., & Zarnecki, A. (2023). Genetic relationships of body condition score and locomotion with production, type and fertility traits in Holstein-Friesian cows. Animal, 17(6), 100816.
Jeong, J. K., & Kim, I. H. (2022). Risk factors for repeat breeder dairy cows and their impacts on reproductive performance. Korean Journal of Veterinary Research, 62(2), e15.
Keshipour, H., Bahonar, A., Vodjgani, M., & Anassori, E. (2024). Effectiveness of training parturition and dystocia management on days open of dairy cows in traditional farming systems: A field trial. Veterinary Research Forum, 15(3), 139–144.
Kgari, R. D., Muller, C. J. C., Dzama, K., & Makgahlela, M. L. (2023). Genetic and phenotypic trends for female fertility traits derived from service records of South African Holstein cattle. South African Journal of Animal Science, 53(4), 598–606.
Kilany, A. A., El-Darawany, A. H. A., EL-Tarabany, A. A., & Al-Marakby, K. M. (2023). Effect of environmental thermal stress on reproductive performance of Holstein cows in Egypt. Biological Rhythm Research, 54(12), 770–781.
Kitade, Y., Tsukano, K., Miyamoto, Y., & Suzuki, K. (2022). Mastitis causes negative reproduction performance similar to genital diseases. Research in Veterinary Science, 153, 35–44.
Kramarenko, A. S., Kalynycnenko, H. I., Susol, R. L., Papakina, N. S., & Kramarenko, S. S. (2022). Principal component analysis of body weight traits and subsequent milk production in Red Steppe Breed Heifers. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, 76(2), 307–313.
Kramarenko, A., Luhovyi, S., Kalynycnenko, H., & Kramarenko, S. (2025). Analysis of lactation length variability and its relationship to cow milk production. Scientific Horizons, 28(3), 9–23.
Kusaka, H., Yamazaki, T., & Sakaguchi, M. (2022). Association of the age and bodyweight at first calving with the reproductive and productive performance in one herd of Holstein dairy heifers in Japan. Veterinary Record Open, 9(1), e44.
Lee, J. G., Seo, J., Alam, M., Song, H., Lee, S., Cho, J., Dang, C. G., & Lee, J. (2024). Estimation of genetic parameters for reproductive traits in Korean dairy cattle. Animal Bioscience, 38(1), 33–40.
Liedgren, S., Fikse, F., Nilsson, K., & Strandberg, E. (2024). Performance of purebred dairy cows and crossbred cows between Swedish Red, Swedish Holstein, Jersey, and Montbéliarde in Swedish herds. Frontiers in Animal Science, 5, 1427014.
Lourenço, J. C. S., Ossani, P. C., Salles, M. S. V., Bánkuti, F. I., Almeida, R. D., Rossoni, D. F., de Osorio, J. A. S., & Santos, G. T. D. (2022). Characterization of dystocia in a herd of Holstein dairy cows in Brazil. Ciência Animal Brasileira, 23, e-72508E.
Macmillan, K., Gobikrushanth, M., Behrouzi, A., Hoff, B., & Colazo, M. G. (2021). Prevalence of early postpartum health disorders in Holstein cows and associations with production, reproduction, and survival outcomes on Alberta dairy farms. The Canadian Veterinary Journal, 62(3), 273–280.
Mahnani, A., Sadeghi-Sefidmazgi, A., Ansari-Mahyari, S., & Ghorbani, G. R. (2021). Assessing the consequences and economic impact of retained placenta in Holstein dairy cattle. Theriogenology, 175, 61–68.
Moawed, S. A., Osman, M. M., Rady, E. A., El-Bayomi, K. M., & Farag, A. F. (2021). Principle component analysis of breeding values estimated by six animal models for evaluating some productive and reproductive traits of Holstein dairy cattle. Advances in Animal and Veterinary Sciences, 9(8), 1113–1122.
Nan, L., Du, C., Fan, Y., Liu, W., Luo, X., Wang, H., Ding, L., Zhang, Y., Chu, C., Li, C., Ren, X., Yu, H., Lu, S., & Zhang, S. (2023). Association between days open and parity, calving season or milk spectral data. Animals, 13(3), 509.
Nasr, M. A., Hussein, M. A., Alkhedaide, A. Q., El-Tarabany, M. S., & Roushdy, E. M. (2021). Reproductive performance and culling rate of purebred Holstein cows and their crosses with Fleckvieh and Brown Swiss cows under subtropical conditions. Frontiers in Veterinary Science, 8, 752941.
Navarro, D. & Foxcroft, D. R. (2025). Learning statistics with JAMOVI: A tutorial for beginners in statistical analysis. Open Book Publishers, Cambridge.
Overton, M. W., & Eicker, S. (2025). Associations between days open and dry period length versus milk production, replacement, and fertility in the subsequent lactation in Holstein dairy cows. Journal of Dairy Science, 108(4), 3764–3779.
Öztürk, Y., & Sipahi, C. (2021). Some fertility traits of Holstein Friesian cattle raised at various production scales in the Western Mediterranean Region of Turkey. Veterinary Journal of Mehmet Akif Ersoy University, 6(3), 148–152.
Polishchuk, T., Karatieieva, E., Bondarenko, V., & Datsuk, I. (2021). The use of dairy cows of French breeding in the conditions of Ukraine. AgroLife Scientific Journal, 10(2), 150–159.
Recce, S., Huber, E., Notaro, U. S., Rodríguez, F. M., Ortega, H. H., Rey, F., Signorini, M. L., & Salvetti, N. R. (2021). Association between heat stress during intrauterine development and the calving-to-conception and calving-to-first-service intervals in Holstein cows. Theriogenology, 162, 95–104.
Reisi-Vanani, R., Ansari-Mahyari, S., Pakdel, A., & Cue, R. I. (2025). Impact of reproductive traits on productive life in Iranian Holstein dairy cows. Journal of Animal Breeding and Genetics, 142(1), 92–101. http://doi.org/10.1111/jbg.12888
Remmik, A., Värnik, R., & Kask, K. (2020). Impact of calving interval on milk yield and longevity of primiparous Estonian Holstein cows. Czech Journal of Animal Science, 65(10), 365–372.
Sadek, R. R., Abou-Bakr, S., Mohamed, A. A. N., Ibrahim, A. E. A. M., Badr, M. M., & Awad, M. A. A. (2021). Evaluation of milk yield and reproductive performance of pure Holstein and its F1 crossbreds with Montbeliarde in Egypt. World's Veterinary Journal, 3, 489–497.
Sahwan, F. M., Elmaghraby, M. M., Ngounndji, A., & Fathalla, M. M. (2021). Factors affecting days postpartum to first service, days open and pregnancy hazard in Holstein cows kept under the subtropical conditions of Egypt. Alexandria Journal of Veterinary Sciences, 71(2), 45–55.
Saranjam, N., Moghaddam, M. F., Akbari, G., Mohammadsadegh, M., & Farzaneh, N. (2020). Associations between milk fat, protein and fat-to-protein ratio with some reproductive indices in dairy cows. Indian Journal of Animal Sciences, 90(5), 764–767.
Sasaki, O., Takeda, H., & Nishiura, A. (2020). The economic value of days open in Holstein cows in Japan based on simulated changes in conception rate. Animal Science Journal, 91(1), e13342.
Silva, H. T., Lopes, P. S., Costa, C. N., Silva, F. F., Silva, D. A., Silva, A. A., Thompson, G., & Carvalheira, J. (2020). Autoregressive repeatability model for genetic evaluation of longitudinal reproductive traits in dairy cattle. Journal of Dairy Research, 87(1), 37–44.
Sokal, R. R., & Rohlf, F. J. (1995). Biometry: The principles and practice of statistics in biological research. Third Edition. Freeman and Co, New York.
Tourchi, J. J., Alijani, S., Rafat, S. A., & Abbasi, M. A. (2024). Bayesian-genome-wide association study and post-GWAS on reproductive traits of Holstein dairy cattle. Journal of Dairy Research, 91(2), 146–155.
Vieira, M. T., Daltro, D. D. S., & Cobuci, J. A. (2022). Breed and heterosis effects on reproduction and production traits of Girolando cows. Revista Brasileira de Zootecnia, 51, e20200266.
Yáñez, U., Álvarez, J., Lorenzo, G., Caínzos, J., Dubuc, J., Becerra, J. J., Herradόn, P. G., Peña, A. I., & Quintela, L. A. (2024). Effect of recombinant bovine granulocyte colony-stimulating factor treatment during the peripartum period on postpartum diseases, reproductive performance, and milk production in Holstein cattle. Research in Veterinary Science, 177, 105368.
Zahed, S. M., Badr, A. A., & Khattab, A. S. (2020). Genetic and phenotypic association between productive and reproductive traits in Friesian cows in Egypt. Journal of Animal and Poultry Production, 11(3), 83–88.
Zahedi, V., Kohram, H., Zeynodini, S., Yousefi, A. R., Baghshahi, H., Moradi-Shahrbabak, M., Zhandi, M., Asad, R. M., & Fouladi-Nashta, A. A. (2021). Trends in reproductive status of Holstein dairy herds in Iran. Iranian Journal of Applied Animal Science, 11(3), 497–505.
Zamorano-Algandar, R., Sánchez-Castro, M. A., Hernández-Cordero, A. I., Enns, R. M., Speidel, S. E., Thomas, M. G., Medrano, J. F., Rincόn, G., Leyva-Corona, J. C., Luna-Nevárez, G., Reyna-Granados, J. R., & Luna-Nevárez, P. (2021). Molecular marker prediction for days open and pregnancy rate in Holstein cows managed in a warm climate. Livestock Science, 250, 104536.
Zargaran, A., Amin Afshar, M., Joezy-Shekalgorabi, S., Azizi, J., & Chamani, M. (2021). Reproductive performance of Holstein heifers inseminated with sex sorted semen in various herd sizes. Iranian Journal of Applied Animal Science, 11(2), 249–259.
Zhu, K., Li, T., Liu, D., Wang, S., Wang, S., Wang, Q., Pan, Y., Zan, L., & Ma, P. (2024). Estimation of genetic parameters for fertility traits in Chinese Holstein of South China. Frontiers in Genetics, 14, 1288375.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons «Attribution» 4.0 License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.


