Inactivated glutathione yeast action on the activity of quails’ antioxidant system

S. Y. Dul; Т. Y. Prudyus; Y. I. Pivtorak; N. А. Broda; І. O. Matiukha; Y. M. Kosenko; V. V. Fedorovych

doi:10.15421/0225183

S. Y. Dul Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies of Lviv
Т. Y. Prudyus Іnstitute of Animal Biology of NAAS
Y. I. Pivtorak Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies of Lviv
N. А. Broda Іnstitute of Animal Biology of NAAS
І. O. Matiukha Enzym Group
Y. M. Kosenko State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives
V. V. Fedorovych Іnstitute of Animal Biology of NAAS

Keywords: quail, inactivated glutathione yeasts, antioxidant system, glutathione, gluthathioneperoxidase, superoxide dismutase, catalase, malondialdehyde, oxidative stress.

Abstract

Antioxidant system is a key element maintaining the metabolic homeostasis in poultry, as it provides the control of a c tive oxygen specie s and prevents damage to cell structures. Disruption of this balance leads to oxidative stress , accomp a nied by intensification of lipid peroxidation, decreased enzyme activity , and reduced productivity. Quails ( Coturnix cotu r nix japonica ) are particularly sensitive to such changes, as they have a high metabolic rate combined with low resistance to zoo technical and environmental stress. In this regard, it is important to find natural products that can maintain antioxidant balance. One of these i s inactivated glutathione yeast. For example, EnzActive Protein Powder is a yeast-based suppl e ment , containing high concentrations of reduced glutathione, peptides, β-glucans, and mannan oligosaccharides, which can activate endogenous antioxidant defen s e mechanisms. The aim of the study was to evaluate the effect of feeding quails with different doses of inactivated glutathione yeast (0.3% and 0.5%) on the activity of antioxidant system enzymes : glut a thione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), the level of reduced glutathione (GSH), and the malondialdehyde (MDA) content. The experiment was conducted on 45 Manchurian quails, which were divided into three groups (control, T1 , T2) according to the analogue principle. Biochemical blood tests were performed on the 14th and 48th days. The results showed that EnzActive Protein Powder promoted an increase in the GPx, SOD, and CAT activit ies by 9.3–17.9%, an increase in the GSH levels by 7.8–14.5%, and a decrease in the MDA concentration by 11.1–19.5% co m pared with the control. The obtained data indicate a stimulating effect of the yeast product on the enzy matic and non enz y matic links of the antioxidant system. The effect was dose-dependent: more changes were observed when using a dos e of 0.5%. Thus, feeding the quails with EnzActive Protein Powder contribute d to the increased antioxidant enzyme activity, stabilization of redox homeostasis, and reduced lipid peroxidation. The results obtained confirm the relevance of using inactivated glutathione yeast as a natural treatment for oxidative stress in intensive poultry farming. Further research pro s pects include studying the effects of inactivated glutathione yeast on performance indicators, immune status, and morph o functional state of the liver in quails.

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