Сomponents of colostrum regulate the activity of the immune system in animals with liver fibrosis

E. G. Ivanov; E. M. Klimova; A. A. Bozhkov; M. K. Kovalova; A. I. Bozhkov

doi:10.15421/0225176

E. G. Ivanov V. N. Karazin National University
E. M. Klimova Zaycev V. T. Institute of General and Urgent Surgery of National Academy of Medical Sciences of Ukraine
A. A. Bozhkov V. N. Karazin National University
M. K. Kovalova V. N. Karazin National University
A. I. Bozhkov V. N. Karazin National University

Keywords: Cu-induced liver fibrosis, low molecular weight protein components of colostrums, LMW, cytotoxic components, DAMPs, immune system, phagocytosis.

Abstract

Regulation of the functional activity of the cellular component of the body's immune system against the background of var i ous liver pathologies, primarily fibrosis, represents a promising approach to developing treatment strategies for such altered fun c tional states. Low molecular weight protein components (LMW) obtained from whole cow colostrum were analysed as natural regulators of immune system activity. The effect of LMW on the number of cytotoxic components in blood serum, the activity of oxygen-dependent and oxygen-independent phagocytosis, and the number of circulating immune complexes in the blood of animals with Cu-induced liver fibrosis was determined. Liver fibrosis was induced in Wistar rats by 6 consecutive injections of copper sulphate at a dose of 1 mg/100 g body weight, and after 3 days one of the groups of animals was administered LMW at a dose of 0.1 mg/100 g body weight per os daily for 6 days. It was found that animals with Cu-induced liver fibrosis had a twofold increase in cytotoxic components in the body compared to the control group. This was accompanied by a twofold increase in the spontaneous level of phagocytic activity of neutrophils (without additional stimulation with zymosan), while the functional r e serve of neutrophils was depleted (there was no stimulation with zymosan); the ability of phagocytic cells to absorb foreign su b stances was increased by only 34%, while the number of circulating immune complexes was reduced by 33%. This immune status of animals with Cu-induced liver fibrosis can be defined as a risk factor for the developmen t of an autoimmune component. Administration of LMW to animals with liver fibrosis for 6 days at a dose of 1 mg/100 g of body weight led to a decrease in phagocytic activity to the level of control indicators, restoration of the reserve of oxygen-dependent phagocytosis of neutrophils and normalisation of oxygen-independent phagocytosis indicators, while the number of circulating immune complexes corre s ponded to the control values. The restoration of cellular parameters under the influence of LMW may prevent the chronic deve l opment of liver fibrosis and its transition to cirrhosis, and further systemic studies will allow the development of treatment tactics using natural LMW.

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