Molecular characteristic of Staphylococcus aureus isolated from patients with nasal carriage infections

A. A. Hammoudi; N. S. Mohammed

doi:10.15421/0225157

A. A. Hammoudi Al-Bayan University
N. S. Mohammed Mustansiriyah University

Keywords: Staphylococcus aureus, icaA gene, icaD gene, antibiotic sensitivity test.

Abstract

Staphylococcus aureus is a path o genic microorganism that lead s to a range of infections in both humans and animals due to its ability to form bi o films an d develop antibiotic resistance. A total of 150 nasal swabs were co l lected from outpatients, from which 52 (34.7%) isolates were identified as S. aureus . Identification of S. aureus isolates was conducted by biochemical analysis ( catalase and coagulase a s says). Biofilm formati on was evaluated using Congo -red agar , and the presence of icaA and icaD genes was confirmed through molecular analysis. Antibiotic sensitivity patterns were assessed by regular antimicrobial testing. All the samples were catalase- and coagulase-positive. Biofilm production was d e tected in 15 (28.8%) specimens. All the samples were icaA and icaD gene- positive. Antibiotic resistance tests confirmed that the highest resistance was exerted against Oxacillin (86.5%), Tetracycline (67.3%), Ciprofloxacin (65.4%), and Erythromycin (63.5%). Vancomycin and Gentamicin demo n strated a strong efficacy. A m oderate efficacy was achieved with Rifampin (96.2%), Cotrimoxazole (94.2%), and Augmentin (90.4%). The present study highlighted the rate of antibiotic resi s tance among nasal S. aureus isolates, including resistance to Oxacillin, which is the gold standard for methicillin-resistant S. aureus (MRSA). These outcomes were further elucidated by positive biofilm genes ( icaA and icaD ), suggesting the pathogenic potential of these isolates. T hese outcomes warrant further study, and effective antibiotic criteria are essential to manage the increasing resistance of S. aureus to ensure the effectiveness of avail a ble therapy.

References

Assafi, M. S., Mohammed, R. Q., & Hussein, N. R. (2015). Nasal carriage rates of Staphylococcus aureus and CA-Methicillin resistant Staphylococcus aureus among university students. Journal of Microbiology Research, 5(4), 123–127.

Atlas, R. M., & Snyder, J. W. (2006). Handbook of media for clinical microbiology. 2nd edition. CRC Press, Boca Raton.

Azmi, K., Qrei, W., & Abdeen, Z. (2019). Screening of genes encoding adhesion factors and biofilm production in methicillin resistant strains of Staphylococcus aureus isolated from Palestinian patients. BMC Genomics, 20(1), 578.

Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493–496.

Biswas, S., Karmakar, A., & Ghosh, C. (2015). Multidrug resistant pathogenic Staphylococcus aureus in the pimples. Medical Science, 16, 41–50.

CLSI (2010). Performance standard for antimicrobial susceptibility testing. Twentieth Informational Supplement. M100-S20.

Cole, A. M., Tahk, S., Oren, A., Yoshioka, D., Kim, Y.-H., Park, A., & Ganz, T. (2001). Determinants of Staphylococcus aureus nasal carriage. Clinical Diagnostic Laboratory Immunology, 8(6), 1064–1069.

Danelli, T., Duarte, F. C., Oliveira, T. A. de, Silva, R. S. da, Frizon Alfieri, D., Gonçalves, G. B., Oliveira, C. F. de, Tavares, E. R., Yamauchi, L. M., Perugini, M. R. E., & Yamada-Ogatta, S. F. (2020). Nasal carriage by Staphylococcus aureus among healthcare workers and students attending a university hospital in Southern Brazil: Prevalence, phenotypic, and molecular characteristics. Interdisciplinary Perspectives on Infectious Diseases, 2020, 3808036.

El-Kady, H. (2015). Nasal carriage of Staphylococcus aureus among paramedical students in Alexandria and evaluation of dry spot staphytect latex kit as a rapid screening method for Staphylococcus aureus. Journal of High Institute of Public Health, 45(2), 52–61.

Fisher, R. A. (1922). On the interpretation of χ2 from contingency tables, and the calculation of P. Journal of the Royal Statistical Society, 85(1), 87–94.

Freeman, D. J., Falkiner, F. R., & Keane, C. T. (1989). New method for detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology, 42(8), 872–874.

Gebremariam, T., & Zelelow, Y. (2014). A systemic review of antimicrobial resistance pattern of methicillin-resistant Staphylococcus aureus. Saudi Journal for Health Sciences, 3(2), 71–74.

Ghellai, L., Hassaine, H., Klouche, N., Khadir, A., Aissaoui, N., Nas, F., & Zingg, W. (2014). Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus isolated in Algeria at the University Hospital of Tlemcen. Journal of Bacteriology Research, 6(1), 1–6.

Hassan, A., Usman, J., Kaleem, F., Omair, M., Khalid, A., & Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. The Brazilian Journal of Infectious Diseases, 15(4), 305–311.

Jain, A., Agarwal, A., & Verma, R. K. (2008). Cefoxitin disc diffusion test for detection of meticillin-resistant staphylococci. Journal of Medical Microbiology, 57(8), 957–961.

Jasim, H. A., & Al-Moosawi, W. N. (2014). Nasal carriage of Staphylococcus aureus among Basra medical students. Basrah Journal of Science, 32(2), 182–193.

Karimi, M., Esfahani, B. N., Halaji, M., Mobasherizadeh, S., Shahin, M., Havaei, S. R., Shokri, D., & Havaei, S. A. (2017). Molecular characteristics and antibiotic resistance pattern of Staphylococcus aureus nasal carriage in tertiary care hospitals of Isfahan, Iran. Le Infezioni in Medicina, 25(3), 234–240.

Karmakar, A., Dua, P., & Ghosh, C. (2016). Biochemical and molecular analysis of Staphylococcus aureus clinical isolates from hospitalized patients. The Canadian Journal of Infectious Diseases and Medical Microbiology, 2016, 9041636.

Khalili, M. B., Sharifi, Y. M. K., Dargahi, H., & Sadeghian, H. A. (2009). Nasal colonization rate of Staphylococcus aureus strains among health care service employee’s of Teaching University Hospitals in Yazd. Acta Medica Iranica, 47(4), 315–317.

Khorvash, F., Abdi, F., Ataei, B., Neisiani, H. F., Kashani, H. H., & Narimani, T. (2012). Nasal carriage of Staphylococcus aureus: Frequency and antibiotic resistance in healthy adults. Journal of Research in Medical Sciences, 17(2), S229–S232.

Kot, B., Sytykiewicz, H., & Sprawka, I. (2018). Expression of the biofilm-associated genes in Methicillin-resistant Staphylococcus aureus in biofilm and planktonic conditions. International Journal of Molecular Sciences, 19(11), 3487.

Kumar, P., Shukla, I., & Varshney, S. (2011). Nasal screening of healthcare workers for nasal carriage of coagulase positive MRSA and prevalence of nasal colonization with Staphylococcus aureus. Biology and Medicine, 3(S2), 182–186.

Mahmood, A. R., & Hussein, N. M. (2022). Detection of biofilm-forming genes in Staphylococcus aureus clinical isolates by PCR assay. Journal of Natural Science, Biology and Medicine, 13(1), 31–40.

Mariana, N. S., Salman, S. A., Neela, V., & Zamberi, S. (2009). Evaluation of modified Congo red agar for detection of biofilm produced by clinical isolates of methicillin resistance Staphylococcus aureus. African Journal of Microbiology Research, 3(6), 330–338.

Mathur, T., Singhal, S., Khan, S., Upadhyay, D. J., Fatma, T., & Rattan, A. (2006). Detection of biofilm formation among the clinical isolates of staphylococci: An evaluation of three different screening methods. Indian Journal of Medical Microbiology, 24(1), 25–29.

McHugh, M. L. (2013). The Chi-square test of independence. Biochemia Medica, 23(2), 143–149.

Munckhof, W. J., Nimmo, G. R., Schooneveldt, J. M., Schlebusch, S., Stephens, A. J., Williams, G., Huygens, F., & Giffard, P. (2009). Nasal carriage of Staphylococcus aureus, including community-associated methicillin-resistant strains, in Queensland adults. Clinical Microbiology and Infection, 15(2), 149–155.

Nester, E. W., Anderson, D. G., Roberts Jr., C. E., & Nester, M. T. (1995). Microbiology: A human perspective. Sixth edition. The McGraw-Hill Companies.

Nour El-Din, H. T., Yassin, A. S., Ragab, Y. M., & Hashem, A. M. (2021). Phenotype-genotype characterization and antibiotic-resistance correlations among colonizing and infectious Methicillin-resistant Staphylococcus aureus recovered from intensive care units. Infection and Drug Resistance, 14, 1557–1571.

Prates, K. A., Torres, A. M., Garcia, L. B., Yamada Ogatta, S. F., Cardoso, C. L., & Bronharo Tognim, M. C. (2010). Nasal carriage of methicillin-resistant Staphylococcus aureus in university students. The Brazilian Journal of Infectious Diseases, 14(3), 316–318.

Ravi, G. C., Shivaprasad, A., Shenoy, P., & Nagaraj, B. T. (2011). Changing pattern of nasal carriage of Staphylococcus aureus in undergraduate medical students. International Journal of Applied Biology and Pharmaceutical Technology, 2(4), 58–63.

Rohde, R. E., Denham, R., & Brannon, A. (2009). Methicillin resistant Staphylococcus aureus: Carriage rates and characterization of students in a Texas University. Clinical Laboratory Science, 22(3), 176–184.

Ryan, K. J., & Ray, C. G. (1984). Sherris medical microbiology: An introduction to infectious diseases. 4th edition. Elsevier, New York, Chicago, San Francisco, Lisbon, London, Madrid, Mexico City, Milan, New Delhi, San Juan, Seoul, Singapore, Sydney, Toronto.

Sivaraman, K., Venkataraman, N., & Cole, A. M. (2009). Staphylococcus aureus nasal carriage and its contributing factors. Future Microbiology, 4(8), 999–1008.

Taj, Y., Essa, F., Aziz, F., & Kazmi, S. U. (2012). Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. Journal of Infection in Developing Countries, 6(5), 403–409.

Tong, S. Y. C., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler, V. G. (2015). Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28(3), 603–661.