Detection of HTLV-1 and -2 infections in relation to the immune factor soluble HLA-G in adult Iraqi lymphoma patients

A. R. H. Al Rawi; Z. A. Hamid; A. S. Naji

doi:10.15421/0225153

A. R. H. Al Rawi University of Baghdad
Z. A. Hamid University of Baghdad
A. S. Naji University of Baghdad

Keywords: Lymphoma, HTLV-1, HTLV-2, soluble human leukocyte antigen-G, TaqMan probe RT-PCR.

Abstract

The o bjective of this article is to determin e the expression of sHLA-G antigen in HTLV-1 and HTLV-2 l ymphoma cases and t o confirm positive infection cases of HTLV-1 and distinguish ra ndom c ases from negative by using the r eal-time polym e rase chain reaction (RT-PCR) technique. This cross-sectional study involved 180 lymphoma patients recruited from the Blood and Bone Marrow Transplantation Center at the Teaching Hospital, Medical City, Baghdad, National Center for Blood Diseases, A l -Mustansiriya University, and A l -Imamain A l -Kadhimain Medical City. A m inority of the participant s were ELISA positive to HTLV-1 . T he same is true for the TaqMan probe RT-PCR result, the mean levels of HLA-G did n o t differ significantly between lymphoma participants who were HTLV-1 positive and those who were HTLV-1 negative. All lymphoma participants in this study were HTLV-2 negative . In this study, lymphoma patients were less predominantly affected by HTLV-1 and HTLV-2 infection. Therefore, it is important to implement screening protocols to control the transmission and complication of this virus in lymphoma patient s . Further research will be necessary , involving all provinces of Iraq , to reveal the exact number of virus infe c tion s .

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