First report on the antibacterial and anti-Fusarium activity of methanolic extract of the red alga Asparagopsis armata

L. Maidi; H. Gouzi; R. Hammoudi

doi:10.15421/0225107

L. Maidi University of Kasdi Merbah Ouargla
H. Gouzi Amar Telidji University
R. Hammoudi University of Kasdi Merbah Ouargla

Keywords: Asparagopsis armata, methanol extract, antibacterial activity, anti-Fusarium activity.

Abstract

This research records the antibacterial and anti- F usarium activit y of the methanolic extract (MeOH) from the marine alga, Asparagopsis armata , collected in Algeria . The antibacterial activity was screened using the w ell diffusion method against five pathogenic bacteria strains: Escherichia coli , Pseudomonas aeruginosa , Bacillus cereus , Klebsiella pneumo nia e and Staphylococcus aureus . The anti- F usarium activity was evaluated using the direct contact method against four Fusarium species ( F . culmorum , F . graminearum , F . poae and F . avenaceum ). The results reve a led that the methanolic extract of A. armata demonstrated notable antibacterial activity against the tested strain s with an inhibition zone ranging between 10 . 2 and 40 .6 mm. The crude methanolic extract of A. armata exhibited significant antibacterial activity against S. aureus , K. pneumoniae and B. cereus with minimal inhibitory concentration ≤ 2 mg/m L , P. aeruginosa appears to be the most resistant strain to the methanolic extract, the MIC > 4.0 mg/m L . C oncentration of 80 mg/m L exhibit ed a total inhibition for all fungal strains except for Fusarium poae. The important activity of the extract was attributed to the volatile compounds such as phenol derivatives, halogenated and terpenoid compounds. The red marine alga Asparagopsis armata represent s an important source of new antibacterial and antifungal agents that can replace the chemical agents used for treatment of human infections and plant diseases.

References

Amimi, A., Mouradi, A., Bennasser, L., & Givernaud, T. (2007). Seasonal variations in thalli and carrageenan composition of Gigartina pistillata (Gmelin) Stackhouse (Rhodophyta, Gigartinales) harvested along the Atlantic coast of Morocco. Phycological Research, 55(2), 143–149.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79.

Bansemir, A., Blume, M., Schröder, S., & Lindequist, U. (2006). Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture, 252(1), 79–84.

Bharathi, D., & Lee, J. (2024). Recent advances in marine-derived compounds as potent antibacterial and antifungal agents: A comprehensive review. Marine Drugs, 22(8), 348.

Bouhlal, R., Riadi, H., & Bourgougnon, N. (2010). Antiviral activity of the extracts of Rhodophyceae from Morocco. African Journal of Biotechnology, 9(46), 7968–7975.

Cadar, E., Popescu, A., Dragan, A.-M.-L., Pesterau, A.-M., Pascale, C., Anuta, V., Prasacu, I., Velescu, B. S., Tomescu, C. L., Bogdan-Andreescu, C. F., Sirbu, R., & Ionescu, A.-M. (2025). Bioactive compounds of marine algae and their potential health and nutraceutical applications: A review. Marine Drugs, 23(4), 152.

Carpena, M., Pereira, C. S. G. P., Silva, A., Barciela, P., Jorge, A. O. S., Perez-Vazquez, A., Pereira, A. G., Barreira, J. C. M., Oliveira, M. B. P. P., & Prieto, M. A. (2024). Metabolite profiling of macroalgae: Biosynthesis and beneficial biological properties of active compounds. Marine Drugs, 22(10), 478.

Carroll, A. R., Copp, B. R., Grkovic, T., Keyzers, R. A., & Prinsep, M. R. (2024). Marine natural products. Natural Product Reports, 41(2), 162–207.

Chakraborty, K., Lipton, A. P., Paul Raj, R., & Vijayan, K. K. (2010). Antibacterial labdane diterpenoids of Ulva fasciata Delile from southwestern coast of the Indian Peninsula. Food Chemistry, 119(4), 1399–1408.

Cheng, S.-S., Liu, J.-Y., Chang, E.-H., & Chang, S.-T. (2008). Antifungal activity of cinnamaldehyde and eugenol congeners against wood-rot fungi. Bioresource Technology, 99(11), 5145–5149.

Choi, J., Shin, D., Kim, M., Park, J., Lim, S., & Ryu, S. (2012). LsrR-mediated quorum sensing controls invasiveness of Salmonella typhimurium by regulating SPI-1 and flagella genes. PLoS One, 7(5), e37059.

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582.

Daglia, M. (2012). Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 23(2), 174–181.

Dembitsky, V. M., & Srebnik, M. (2002). Natural halogenated fatty acids: Their analogues and derivatives. Progress in Lipid Research, 41(4), 315–367.

Devi, G. K., Manivannan, K., Thirumaran, G., Rajathi, F. A. A., & Anantharaman, P. (2011). In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pacific Journal of Tropical Medicine, 4(3), 205–211.

El Omari, F., Mouradi, A., Bennaser, L., Bennis, M., Blail, H., Mouradi, A., & Givernaud, T. (2011). Analyse de la croissance de Gymnogongrus patens Agardh de la côte atlantique marocaine. Afrique Science, 3(3), 102–119.

Etahiri, S., Bultel-Poncé, V., Caux, C., & Guyot, M. (2001). New bromoditerpenes from the red alga Sphaerococcus coronopifolius. Journal of Natural Products, 64(8), 1024–1027.

Etahiri, S., El Kouria, A. K., Bultel-Poncé, V., Guyot, M., & Assobhei, O. (2007). Antibacterial bromophenol from the marine red alga Pterosiphonia complanata. Natural Product Communications, 2(7), 749–752.

Genovese, G., Tedone, L., Hamann, M. T., & Morabito, M. (2009). The Mediterranean red alga Asparagopsis: A source of compounds against Leishmania. Marine Drugs, 7(3), 361–366.

Goodwin, K. D., North, W. J., & Lidstrom, M. E. (1997). Production of bromoform and dibromomethane by Giant Kelp: Factors affecting release and comparison to anthropogenic bromine sources. Limnology and Oceanography, 42(8), 1725–1734.

Grimes, S., Benabdi, M., Babali, N., Refes, W., Boudjellal-Kaidi, N., & Seridi, H. (2018). Biodiversity changes along the Algerian coast (Southwest Mediterranean basin): From 1834 to 2017: A first assessment of introduced species. Mediterranean Marine Science, 19(1), 156–179.

Hassen, B. M., Rashedy, S. H., Mostafa, A., Mahrous, N., Nafie, M. S., Elebeedy, D., & Abdel Azeiz, A. Z. (2023). Identification of potential antiviral compounds from Egyptian marine algae against influenza A virus. Natural Product Research, 38(24), 4411–4418.

Hmani, I., Ktari, L., Ismail, A., M’dallel, C., & El Bour, M. (2021). Assessment of the antioxidant and antibacterial properties of red algae (Rhodophyta) from the north coast of Tunisia. Euro-Mediterranean Journal for Environmental Integration, 6(1), 13.

Horta, A., Alves, C., Pinteus, S., Lopes, C., Fino, N., Silva, J., Ribeiro, J., Rodrigues, D., Francisco, J., Rodrigues, A., & Pedrosa, R. (2019). Identification of Asparagopsis armata-associated bacteria and characterization of their bioactive potential. MicrobiologyOpen, 8(11), e824.

Ibrahim, D., & Lim, S.-H. (2015). In vitro antimicrobial activities of methanolic extract from marine alga Enteromorpha intestinalis. Asian Pacific Journal of Tropical Biomedicine, 5(9), 785–788.

Jacinto, M. S., Monteiro, H. R., & Lemos, M. F. (2013). Impact of the invasive macroalgae Asparagopsis armata on coastal environments: An ecotoxicological assessment. Current Opinion in Biotechnology, 24, S75.

Kladi, M., Vagias, C., & Roussis, V. (2004). Volatile halogenated metabolites from marine red algae. Phytochemistry Reviews, 3(3), 337–366.

Kotan, R., Kordali, S., Cakir, A., Kesdek, M., Kaya, Y., & Kilic, H. (2008). Antimicrobial and insecticidal activities of essential oil isolated from Turkish Salvia hydrangea DC. ex Benth. Biochemical Systematics and Ecology, 36(5–6), 360–368.

Ktari, L., & Guyot, M. (1999). A cytotoxic oxysterol from the marine alga Padina pavonica (L.) Thivy. Journal of Applied Phycology, 11(6), 511–513.

Küpper, F. C., Schweigert, N., Ar Gall, E., Legendre, J.-M., Vilter, H., & Kloareg, B. (1998). Iodine uptake in Laminariales involves extracellular, haloperoxidase-mediated oxidation of iodide. Planta, 207(2), 163–171.

Lee, K., Heo, S. H., Lee, J., Park, S. I., Kim, M., & Sam, S. M. (2020). Antimicrobial, antioxidative, elastase and tyrosinase inhibitory effect of supercritical and hydrothermal Asparagopsis armata extract. International Journal of Advanced Culture Technology, 8(3), 231–240.

Lourenço-Lopes, C., Fraga-Corral, M., Jimenez-Lopez, C., Pereira, A. G., Garcia-Oliveira, P., Carpena, M., Prieto, M. A., & Simal-Gandara, J. (2020). Metabolites from macroalgae and its applications in the cosmetic industry: A circular economy approach. Resources, 9(9), 101.

Mahizan, N. A., Yang, S.-K., Moo, C.-L., Song, A. A.-L., Chong, C.-M., Chong, C.-W., Abushelaibi, A., Lim, S.-H. E., & Lai, K.-S. (2019). Terpene derivatives as a potential agent against antimicrobial resistance (AMR) pathogens. Molecules, 24(14), 2631.

Manso, T., Lores, M., & de Miguel, T. (2021). Antimicrobial activity of polyphenols and natural polyphenolic extracts on clinical isolates. Antibiotics, 11(1), 46.

McConnell, O., & Fenical, W. (1977). Halogen chemistry of the red alga Asparagopsis. Phytochemistry, 16(3), 367–374.

Messahli, I., Gouzi, H., Sifi, I., Chaibi, R., Rezzoug, A., & Rouari, L. (2022). Anticandidal activity of dichloromethane extract obtained from the red algae A. armata of the Algerian Coast. Acta Ecologica Sinica, 42(5), 461–466.

Paul, N., de Nys, R., & Steinberg, P. (2006). Chemical defence against bacteria in the red alga Asparagopsis armata: linking structure with function. Marine Ecology Progress Series, 306, 87–101.

Pinteus, S., Alves, C., Monteiro, H., Araújo, E., Horta, A., & Pedrosa, R. (2015). Asparagopsis armata and Sphaerococcus coronopifolius as a natural source of antimicrobial compounds. World Journal of Microbiology and Biotechnology, 31(3), 445–451.

Pinteus, S., Lemos, M. F. L., Simões, M., Alves, C., Silva, J., Gaspar, H., Martins, A., Rodrigues, A., & Pedrosa, R. (2020). Marine invasive species for high-value products’ exploration – Unveiling the antimicrobial potential of Asparagopsis armata against human pathogens. Algal Research, 52, 102091.

Saim, S., Sahnouni, F., Bouhadi, D., & Kharbouche, S. (2021). The antimicrobial activity of two marine red algae collected from Algerian West Coast. Trends in Pharmaceutical Sciences and Technologies, 7(4), 233–242.

Tasdemir, D., Bugni, T., Lindsay, B., James, R., Copp, B., Ireland, C., Kay Harper, M., VanWagoner, R., Verbitski, S., Richardson, A., & Schnabel, P. (2001). Introduction to the chemical ecology of marine natural products. In: McClintock, J. B., & Baker, B. J. (Eds.). Marine chemical ecology. CRC Press, Boca Raton. pp. 3–69.

Wang, T., Jónsdóttir, R., & Ólafsdóttir, G. (2009). Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds. Food Chemistry, 116(1), 240–248.

Wiegand, I., Hilpert, K., & Hancock, R. E. W. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3(2), 163–175.

Wright, A. D., König, G. M., Angerhofer, C. K., Greenidge, P., Linden, A., & Desqueyroux-Faúndez, R. (1996). Antimalarial activity: The search for marine-derived natural products with selective antimalarial activity. Journal of Natural Products, 59(7), 710–716.

Yin, M., & Tsao, S. (1999). Inhibitory effect of seven Allium plants upon three Aspergillus species. International Journal of Food Microbiology, 49(1–2), 49–56.

Zanolla, M., Carmona, R., Mata, L., De la Rosa, J., Sherwood, A., Barranco, C. N., Muñoz, A. R., & Altamirano, M. (2022). Concise review of the genus Asparagopsis Montagne, 1840. Journal of Applied Phycology, 34, 1–17.

Zinedine, A., Elakhdari, S., Faid, M., & Benlemlih, M., (2004). Antifungal and antiaflatoxinogenic activity of the brown algae Cystoseira tamariscifolia. Journal de Mycologie Medicale, 14(4), 201–205.

Zubia, M., Robledo, D., & Freile-Pelegrin, Y. (2007). Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. Journal of Applied Phycology, 19(5), 449–458.

Zuorro, A., Lavecchia, R., Contreras-Ropero, J. E., Martínez, J. B. G., Barajas-Ferreira, C., & Barajas-Solano, A. F. (2024). Natural antimicrobial agents from algae: Current advances and future directions. International Journal of Molecular Sciences, 25(21), 11826.