Asseesment of the microbiological, physicochemical quality of Diyala River water, with emphasis on regulatory roles of coliform bacteria

  • M. A. Fahad Middle Technical University
  • N. A. Jasim Middle Technical University
  • A. I. Naif Diyala Education Directorate
Keywords: coliform bacteria, microbial pollution, fecal contamination, water pollution, bacterial contamination.

Abstract

Surface water bodies are essential sources of water for human consumption, residential use, agriculture, industrial operations, as well as for aquatic life and ecosystems. The a im of this article is to appraise the microbiological, physical, and chemical quality of the water of the Diyala River . Water samples we re collected from nine various sites along the Diyala River, during the period from winter (December ) 2023 to summer (May ) 2024. The total count of aerobic bacteria, total coliforms (TC) and fecal coliforms (FC), E. coli bacteria and physico-chemical parameters are evaluated. The results show that the water from the Diyala River is contaminated with coliform organisms in all sites. Regarding pathogens, which include bacteria Salmonella spp., Shigella spp., Klebsiella pneumoniae , Enterobacter ludwigii , and Staphylococcus spp., the results specify that there is no clear difference in abu n dance of pathogens between the two seasons. The m ean , of maximum and minimum value for temperature, electrical conduction, turbidity , BOD, DO, calcium , magnesium , n itrate concentration are all greater than the permitted values of the World Health O r ganization ( WHO ) . However, pH, chloride concentration and total dissolved solids ( TDS ) in the water samples are all within the permitted levels set by the WHO. The water quality index (WQI) and comprehensive pollution i ndex (CPI) score for all sites ind i cate that the river water quality is poor. The study indicates that the water in the study area is heavily polluted and poses significant health risks because of fecal and emerging pathogens in the samples. Therefore, there is a need for an immediate treatment for reuse.

References

Alsarayreh, A. A., Al-Obaidi, M. A., Al-Hroub, A. M., Patel, R., & Mujtaba, I. M. (2020). Performance evaluation of reverse osmosis brackish water desalination plant with different recycled ratios of retentate. Computers and Chemical Engineering, 135, 106729.

Attia, M. M., Mosallam, T., Samir, O., Ali, A., & Samir, A. (2023). Toxascaris leonina infected domestic cat (Felis catus) in Egypt; PCR-based molecular characterization of nematode eggs: A potential hazards to human health. Journal of Parasitic Diseases, 47(4), 843–849.

Awad, A., Hassoon, S., Ali, H., & Mohammad, S. (2025). Cultural and biochemical identification of antibiotic-resistant bacteria in the ears of dogs and patients with otitis externa. Archives of Veterinary Medicine, 18(1), 51–70.

Bhutiani, R., Ahamad, F., Tyagi, V., & Ram, K. (2018). Evaluation of water quality of River Malin using water quality index (WQI) at Najibabad, Bijnor (UP) India. Environment Conservation Journal, 19(1–2), 191–201.

Bijay-Singh, & Craswell, E. (2021). Fertilizers and nitrate pollution of surface and ground water: An increasingly pervasive global problem. SN Applied Sciences, 3(4), 518.

Chaudhry, F. N., & Malik, M. F. (2017). Factors affecting water pollution: A review. Journal of Ecosystem and Ecography, 7(1), 225–231.

Chowdhary, P., Bharagava, R. N., Mishra, S., & Khan, N. (2019). Role of industries in water scarcity and its adverse effects on environment and human health. In: Shukla, V., & Kumar, N. (Eds.). Environmental concerns and sustainable development. Volume 1: Air, water and energy resources. Springer, Singapore. Pp. 235–256.

Essa, S. H., Mohammad, S. Q., Kadhum, D. A., & Jalil, I. S. (2024). Effectiveness of silver nanoparticles as antibacterial agents with natural wound healing cream with extracted Aloe vera gel. Regulatory Mechanisms in Biosystems, 15(4), 821–825.

Hadi, N. S. (2023). Evaluation of water and sediment quality by bacteriological diversity studies on certain locations of the Diyala River, Baghdad. Environment and Natural Resources Journal, 21(6), 1–10.

Hassan, F. M., Al-Jibouri, K. D. W., & Hakman, A. A. (2017). Water quality assessment of Diyala River in Diyala Province, Iraq. Mesopotamia Environmental Journal, 4(1), 52–61.

Kadhum, D. A., Al-Qaisi, S. K., Mohammad, S. Q., & Jalil, I. S. (2025). Assessing the genetic basis of antibiotic resistance in Escherichia coli through advanced electrophoretic and PCR techniques. Regulatory Mechanisms in Biosystems, 33(1), e25036.

Kaur, H., Katyal, P., & Chandel, S. (2022). Assessment of water quality using different physicochemical and biological parameters: A case study of Buddha Nallah, Punjab, India. Environment Conservation Journal, 23(3), 145–159.

Kılıç, Z. (2020). The importance of water and conscious use of water. International Journal of Hydrology, 4(5), 239–241.

Köster, W., Egli, T., Ashbolt, N., Botzenhart, K., Burlion, N., Endo, T., … Rust, A. (2003). Analytical methods for microbiological water quality testing. In: OECD & World Health Organization (Eds.). Assessing microbial safety of drinking water. Improving approaches and methods. Pp. 237–295.

Kumar, V., Sahu, P., Singh, P. K., & Markandeya (2020). Multivariate statistical approach for the analysis of organic and inorganic pollutants loads in gomti river at Lucknow City. International Journal of Environmental Research, 14(6), 653–666.

Malik, A. W., Mohsen, S. M., Mohsen, A. K., Mohammad, S. Q., & Mohsein, O. A. (2025). Bacterial strains isolated from sinusitis infections and their drug resistance profiles in Nasiriyah (Iraq). Regulatory Mechanisms in Biosystems, 16(2), e25049.

Mao, G., Hu, H., Liu, X., Crittenden, J., & Huang, N. (2021). A bibliometric analysis of industrial wastewater treatments from 1998 to 2019. Environmental Pollution, 275, 115785.

Mishra, S., Sharma, M. P., & Kumar, A. (2016). Assessment of surface water quality in Surha Lake using pollution index, India. Journal of Material and Environmental Science, 7, 713–719.

Mohammad, S. Q., Awayid, H. S., & Zarrouk-Mahjoub, S. (2025). Identification of virulence genes from clinical isolates of Methicillin-resistant Staphylococcus aureus (MRSA). Biodiversitas Journal of Biological Diversity, 25(12), 5083–5093.

Mohammad, S. Q., Awayid, H. S., Zarrouk-Mahjoub, S., & Jallil, I. S. (2025). Molecular identification of erm A and erm B, erm C genes in Methicillin-resistant Staphylococcus aureus isolates from burns patients and their association with multidrug resistance. Cellular and Molecular Biology, 71(5), 112–117.

Oblinger, J. L., & Koburger, J. A. (1975). Understanding and teaching the most probable number technique 1. Journal of Milk and Food Technology, 38(9), 540–545.

Othman, B. (2020). The Influence of total quality management on competitive advantage towards bank organizations: Evidence from Erbil/Iraq. International Journal of Psychosocial Rehabilitation, 24(5), 3427–3439.

Pham, H. N. (2020). Relative water quality index (ReWQI) – a new method for aggregate water quality assessment. Water and Environment Journal, 34(S1), 873–883.

Pramanik, A. K., Majumdar, D., & Chatterjee, A. (2020). Factors affecting lean, wet-season water quality of Tilaiya Reservoir in Koderma District, India during 2013–2017. Water Science, 34(1), 85–97.

Purgar, M., Gavrilović, A., Kapetanović, D., Klanjšček, J., Jug-Dujaković, J., Kolda, A., Žunić, J., Kazazić, S., Vardić Smrzlić, I., Vukić Lušić, D., Pikelj, K., Listeš, E., El-Matbouli, M., Lillehaug, A., Lončarević, S., Knežević, D., Hengl, B., Geček, S., & Klanjscek, T. (2023). Assessment of Vibrio spp. abundance as a water quality indicator: Insights from Mali Ston Bay in the Adriatic Sea. Estuarine, Coastal and Shelf Science, 295, 108558.

Qassim Mohammad, S., Khazal Mohsen, A., Saleh Jalil, I., & Akram Mohsein, O. (2024). Assessing the prevalence of bacterial vaginosis among infertile women in Thi-Qar Province, Iraq. Infection Epidemiology and Microbiology, 10(4), 287–296.

Saari, G. N., Wang, Z., & Brooks, B. W. (2017). Revisiting inland hypoxia: Diverse exceedances of dissolved oxygen thresholds for freshwater aquatic life. Environmental Science and Pollution Research, 25(4), 3139–3150.

Sharma, R., Kumar, R., Satapathy, S. C., Al-Ansari, N., Singh, K. K., Mahapatra, R. P., Agarwal, A. K., Le, H. V., & Pham, B. T. (2020). Analysis of water pollution using different physicochemical parameters: A study of Yamuna River. Frontiers in Environmental Science, 8, 581591.

Some, S., Mondal, R., Mitra, D., Jain, D., Verma, D., & Das, S. (2021). Microbial pollution of water with special reference to coliform bacteria and their nexus with environment. Energy Nexus, 1, 100008.

Song, Y., Qi, J., Deng, L., Bai, Y., Liu, H., & Qu, J. (2021). Selection of water source for water transfer based on algal growth potential to prevent algal blooms. Journal of Environmental Sciences, 103, 246–254.

Sood, A., Singh, K. D., Pandey, P., & Sharma, S. (2008). Assessment of bacterial indicators and physicochemical parameters to investigate pollution status of Gangetic River system of Uttarakhand (India). Ecological Indicators, 8(5), 709–717.

World Health Organization (2021). Global patient safety action plan 2021–2030: Towards eliminating avoidable harm in health care. World Health Organization.

Published
2025-10-31
How to Cite
Fahad, M. A., Jasim, N. A., & Naif, A. I. (2025). Asseesment of the microbiological, physicochemical quality of Diyala River water, with emphasis on regulatory roles of coliform bacteria. Regulatory Mechanisms in Biosystems, 16(3), e25093. https://doi.org/10.15421/0225093