Raw material base for the production of food and pharmacological glycerin
Abstract
This paper presents a comprehensive analytical review of feedstock sources for the production of food-grade and pharma ceutical glycerin, an important component in a wide range of products. The study covers six key feedstock categories: vegetable oils (palm, soybean, rapeseed, coconut), animal fats (beef, pork, poultry), biodiesel by-products, petrochemical feedstock (propylene), food industry waste, and microbiological synthesis using strains of Yarrowia lipolytica and Candida krusei . The results indicate that glycerin content ranges from 5–15% in natural sources to 40–85% in crude glycerol derived from biodiesel. The chemical compos i tion of various types of raw materials was investigated, and the main groups of impurities were identified, particularly fatty acids, methanol, salts, catalysts, phospholipids, sulfur-containing compounds, and heavy metals, which significantly affect the quality of the final product. A comparative analysis of technological approaches to raw material processing was conducted, including alkaline and enzymatic hydrolysis, transesterification, and the synthetic method, considering glycerin yield, the purity of the final product, energy consumption, and environmental impact. Glycerin purification methods were systematized, including vacuum distillation, ion exchange, adsorption, and membrane filtration, and their effectiveness in achieving pharmacopoeial purity standards (99.5%) was assessed. A critical analysis of modern scientific research identified promising areas for developing the raw material base, particularly the use of selective adsorbents for purification, integration of biodiesel and glycerin production, hydrolysis technologies, hybrid membrane methods, and microbiological synthesis based on waste. Economic analysis showed that the cost of glycerin obtained as a by-product of biodiesel production is 40–60% lower compared to its synthetic counterpart. However, the need for additional purification to meet pharmacopoeial quality standards reduces overall profitability by 15–25%. The practical significance of this study lies in its potential to help manufacturers find optimal sources of raw materials with high glycerin content to ensure the quality of the final product. Additionally, this study will facilitate the development of appropriate technological solutions for the optimal process of glycerin production from selected raw materials.References
Agarwal, P., & Mishra, V. (2023). Multi-criteria decision analysis for selecting optimal glycerin sources: A fuzzy-analytical hierarchy process approach. Journal of Cleaner Production, 398, 136578.
Ahmad, F. B., Zhang, Z., Doherty, W. O., & O'Hara, I. M. (2019). The outlook of producing advanced fuels and chemicals from integrated oil palm biomass biorefinery. Renewable and Sustainable Energy Reviews, 109, 386–411.
Ahmed, S., Khan, R., & Sharma, D. (2022). Cost reduction strategies for pharmaceutical-grade glycerin: Integration of purification processes at the biodiesel production stage. Chemical Engineering Research and Design, 186, 129–142.
Alvarez-Mateos, P., García-Martín, J. F., Guerrero-Vacas, F. J., Naranjo-Calderón, C., Barrios, C. C., & Pérez-Camino, M. C. (2019). Valorization of a high-acidity residual oil generated in the waste cooking oils recycling industries. Grasas y Aceites, 70(4), e335.
Anitha, M., Kamarudin, S. K., & Kofli, N. T. (2016). The potential of glycerol as a value-added commodity. Chemical Engineering Journal, 295, 119–130.
Ayoub, M., & Abdullah, A. Z. (2012). A critical review of the current scenario and significance of crude glycerol resulting from the biodiesel industry towards a more sustainable renewable energy industry. Renewable and Sustainable Energy Reviews, 16(5), 2671–2686.
Bagheri, S., Julkapli, N. M., & Yehye, W. A. (2015). Catalytic conversion of biodiesel-derived raw glycerol to value-added products. Renewable and Sustainable Energy Reviews, 41, 113–127.
Bagnato, G., Iulianelli, A., Sanna, A., & Basile, A. (2017). Glycerol production and transformation: A critical review with particular emphasis on glycerol reforming reaction for producing hydrogen in conventional and membrane reactors. Membranes, 7(2), 17.
Barbirato, F., Himmi, E. H., Conte, T., & Bories, A. (1998). 1,3-propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries. Industrial Crops and Products, 7(2–3), 281–289.
Choudhary, S., Kumar, A., & Singh, R. K. (2024). Sustainability assessment of biodiesel-derived glycerol valorization pathways: A comprehensive review. Renewable and Sustainable Energy Reviews, 185, 113675.
Ciriminna, R., Pina, C. D., Rossi, M., & Pagliaro, M. (2014). Understanding the glycerol market. European Journal of Lipid Science and Technology, 116(10), 1432–1439.
Davis, R., Kataria, R., Cerrone, F., Woods, T., Kenny, S., O'Donovan, A., Guzik, M., Shaikh, H., Duane, G., Gupta, V. K., Tuohy, M. G., Padamatti, R. B., Casey, E., & O'Connor, K. E. (2018). Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains. Bioresource Technology, 150, 202–209.
Fan, X., Burton, R., & Zhou, Y. (2010). Glycerol (a byproduct of biodiesel production) as a source for fuels and chemicals – Mini review. The Open Fuels and Energy Science Journal, 3(1), 17–22.
Foster, W. R., Thompson, R. L., & Nakamura, D. (2022). Economic modeling of glycerol production from diverse feedstocks. Bioresource Technology Reports, 18, 100982.
Galadima, A., & Muraza, O. (2014). Catalytic upgrading of glycerol to fuel additives over supported metal catalysts: A review. Journal of the Taiwan Institute of Chemical Engineers, 45(5), 2343–2354.
González-Pajuelo, M., Meynial-Salles, I., Mendes, F., Andrade, J. C., Vasconcelos, I., & Soucaille, P. (2005). Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metabolic Engineering, 7(5–6), 329–336.
Harris, J. W., Weiland, R. H., Parker, M. E., & Vaghjiani, J. D. (2023). Life cycle assessment of glycerol production from waste streams. Journal of Cleaner Production, 380, 134845.
Herrera-González, A., Martínez-Alonso, E., & Sánchez-Castro, I. (2019). Gas chromatography-mass spectrometry method for the detection of trace contaminants in glycerol from various origins. Analytica Chimica Acta, 1076, 91–102.
Ibrahim, S., Rahman, M., & Omar, R. (2020). Novel selective adsorbents for purification of crude glycerin: Optimization and characterization. Separation and Purification Technology, 242, 116782.
Iwasaki, Y., Sawada, T., & Nakayama, K. (2020). Comprehensive-dimensional liquid chromatography coupled with mass spectrometry for detailed characterization of glycerol-derived impurities in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis, 182, 113138.
Jain, S., & Patel, N. (2021). Pharmacopoeial specifications and analytical methods for glycerin: A comparative review of global pharmacopeias. Journal of Pharmaceutical Analysis, 11(5), 680–690.
Jitchaiyapoom, T., Panjapornpon, C., Bardeeniz, S., & Hussain, M. A. (2024). Production capacity prediction and optimization in the glycerin purification process: A simulation-assisted few-shot learning approach. Processes, 12(4), 661.
Johnson, E. T., Phillips, K. M., & Brown, A. (2024). Decision-making algorithm for optimal glycerin feedstock selection under fluctuating market conditions: Econometric modeling approach. Journal of Cleaner Production, 429, 139871.
Kato, Y., Yamamoto, H., & Suzuki, T. (2021). Technological integration in biodiesel and glycerin production: Process intensification strategies. Fuel, 290, 119851.
Kaushik, N., & Reddy, P. (2023). Molecular mechanisms of glycerin degradation during long-term storage: Impact of residual catalysts and oxidizing agents. Journal of the American Oil Chemists' Society, 100(5), 1047–1061.
Kim, J., Lee, H., & Park, C. (2024). Advanced hybrid membrane technology for continuous glycerin purification: Integrating ultrafiltration, nanofiltration, and ion exchange. Journal of Membrane Science, 669, 121728.
Kim, Y. H., Park, S. M., & Lee, J. S. (2021). Long–term stability studies of glycerol from various sources. Journal of Industrial and Engineering Chemistry, 97, 401–409.
Kolyanovska, L., Palamarchuk, I., Sukhenko, Y., Mussabekova, A., Bissarinov, B., Popiel, P., Mushtruk, M., Sukhenko, V., Vasuliev, V., Semko, T., & Tyshchenko, L. (2019). Mathematical modeling of the extraction process of oil-containing raw materials with pulsed intensification of heat of mass transfer. In: Romaniuk, R. S., Smolarz, A., & Wójcik, W. (Eds.). Optical fibers and their applications 2018. 18th Conference on optical fibers and their applications. Spie. P. 25.
Kongjao, S., Damronglerd, S., & Hunsom, M. (2010). Purification of crude glycerol derived from waste used-oil methyl ester plant. Korean Journal of Chemical Engineering, 27(3), 944–949.
Kowalski, Z., Kulczycka, J., & Kąkol, M. (2020). Economic and environmental assessment of advanced materials for glycerol purification in the context of the circular economy. Journal of Cleaner Production, 252, 119833.
Koyama, T., Nakamura, S., & Tanaka, K. (2022). High-performance liquid chromatography with refractive index detection for simultaneous determination of glycerol and related polyols in biodiesel products. Journal of Separation Science, 45(8), 1457–1466.
Li, J., Wang, T., & Zhang, X. (2021). Limitations of mathematical models for glycerol purification from biodiesel waste. Industrial and Engineering Chemistry Research, 60(3), 1289–1296.
Li, J., Wang, X., Klein, M. T., & Bhatia, V. (2019). Catalytic distillation of glycerin for production of acetol and polyglycerols. Chemical Engineering Science, 174, 179–188.
Liu, Y., Zhang, W., & Chen, J. (2023). Functionalized zeolites for selective adsorption of contaminants from crude glycerin: Achieving 98.5% removal efficiency. Chemical Engineering Journal, 456, 140676.
Lu, J., Umeshita, T., Sakai, S., Matsuo-Ueda, M., Ando, D., Chen, S., & Umemura, K. (2025). Potential of glycerol as a bio-based wood adhesive. Journal of Wood Science, 71(1), 4.
Luo, Z. G., Peng, L., & Liu, L. Y. (2021). NMR spectroscopic analysis of glycerol produced by enzymatic hydrolysis. Journal of Analytical Methods in Chemistry, 2021, 9867410.
Manosak, R., Limpattayanate, S., & Hunsom, M. (2011). Sequential-refining of crude glycerol derived from waste used-oil methyl ester plant via a combined process of chemical and adsorption. Fuel Processing Technology, 92(1), 92–99.
Markovski, J., Smith, A. D., & Thompson, B. J. (2024). Life cycle assessment of pharmaceutical glycerol: From diverse feedstocks to end-use applications. Journal of Cleaner Production, 423, 139380.
Martinez, P., Garcia, J., & Fernandez, L. (2021). Techno-economic analysis of glycerol purification processes for pharmaceutical applications. Chemical Engineering Research and Design, 171, 242–256.
Martinez, T. H., Wilson, J. S., & Thompson, R. L. (2020). Kinetic limitations of catalyst removal in biodiesel-derived glycerol. Chemical Engineering Science, 215, 115424.
Mendes, A. C., Santos, F. D., & Vieira, R. P. (2023). Advanced chromatographic methods for trace analysis of impurities in pharmaceutical-grade glycerol. Journal of Chromatography A, 1695, 463901.
Mendes, A. C., Santos, F. D., & Vieira, R. P. (2023). Advanced chromatographic methods for trace analysis of impurities in pharmaceutical-grade glycerol. Journal of Chromatography A, 1695, 463901.
Mushtruk, M., Bal-Prylypko, L., Slobodyanyuk, N., Boyko, Y., Nikolaienko, M. (2022). Design of reactors with mechanical mixers in biodiesel production. In: Ivanov, V., Pavlenko, I., Liaposhchenko, O., Machado, J., Edl, M. (Eds.). Advances in design, simulation and manufacturing V. DSMIE 2022. Lecture notes in mechanical engineering. Springer, Cham.
Mushtruk, M., Deviatko, O., Ulianko, S., Kanivets, N., Mushtruk, N. (2021). An agro-industrial complex fat-containing wastes synthesis technology in ecological biofuel. In: Ivanov, V., Pavlenko, I., Liaposhchenko, O., Machado, J., Edl, M. (Eds.). Advances in design, simulation and manufacturing IV. DSMIE 2021. Lecture notes in mechanical engineering. Springer, Cham.
Mushtruk, M., Mushtruk, N., Slobodyanyuk, N., Vasyliv, V., & Zheplinska, M. (2024). Enhanced energy independence: Converting animal fat into biodiesel. International Journal of Environmental Studies, 81(1), 134–144.
Mushtruk, M., Vasyliv, V., Slobodaniuk, N., Mukoid, R., & Deviatko, O. (2020). Improvement of the production technology of liquid biofuel from technical fats and oils. In: Ivanov, V., Pavlenko, I., Liaposhchenko, O., Machado, J., & Edl, M. (Eds.). Advances in design, simulation and manufacturing III. DSMIE 2020. Lecture notes in mechanical engineering. Springer, Cham.
Nagata, H., Takahashi, K., & Kobayashi, T. (2021). Comprehensive profiling of glycerol-related compounds in pharmacopoeial-grade glycerin using supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 201, 114100.
Nguyen, T. D., Park, J. Y., & Kim, S. B. (2022). Market analysis and economic assessment of glycerol production from various sources: Challenges and opportunities in the current landscape. Biomass and Bioenergy, 159, 106411.
Nguyen, T. H., Pham, V. T., & Lee, J. W. (2024). Engineered microbial platforms for sustainable glycerol production: Progress and challenges. Biotechnology Advances, 65, 108292.
Okoye, P. U., & Hameed, B. H. (2016). Review on recent progress in catalytic carboxylation and acetylation of glycerol as a byproduct of biodiesel production. Renewable and Sustainable Energy Reviews, 53, 558–574.
Park, S. M., Chen, H., & Liu, Y. (2022). Hybrid membrane technology for continuous glycerol purification. Separation and Purification Technology, 289, 120730.
Peng, L., Zhang, H., & Liu, Y. (2021). Development and validation of a rapid HPLC–MS/MS method for quantitative analysis of glycerol and glycerindes in oils and biodiesel. Analytical Methods, 13(4), 501–510.
Peterson, E. M., Richardson, D. L., & Campbell, J. A. (2024). Novel membrane-based processes for high-purity glycerol recovery from diverse waste streams. Separation and Purification Technology, 324, 124563.
Roberts, T. H., Wilson, J. S., & Johnson, D. T. (2021). Standardized methods for glycerol quality assessment. Journal of the American Oil Chemists' Society, 98(7), 753–763.
Rodrigues, A., Pereira, M., & Oliveira, S. (2023). Cost-benefit analysis of integrated biodiesel-glycerol production facilities: A case study from Brazil. Renewable Energy, 198, 1235–1247.
Santibañez, C., Varnero, M. T., & Bustamante, M. (2024). Economic assessment of glycerol production from waste cooking oil: Implications for circular economy in developing countries. Journal of Environmental Management, 289, 112509.
Schmidt, L. D., Davis, R. J., & Joly, J. F. (2020). Limitations of animal fats as a source of pharmaceutical glycerol. Journal of Pharmaceutical Sciences, 109(4), 1587–1596.
Sułkowski, W. W., Wolińska, A., Szoltysik, M., & Bajdur, W. M. (2018). The influence of very low temperature on the structure and properties of glycerol. Journal of Molecular Structure, 1157, 382–388.
Sun, D., Yamada, Y., Sato, S., & Ueda, W. (2016). Glycerol hydrogenolysis into useful C3 chemicals. Applied Catalysis B: Environmental, 193, 75–92.
Thompson, R. L., Davis, R. J., & Smith, J. K. (2019). Life cycle assessment of glycerol production technologies. Bioresource Technology Reports, 8, 100330.
Turner, T. L., Kim, H., Fry, M., & Layton, D. S. (2022). Economic challenges in pharmaceutical glycerol production from biodiesel waste. Biofuels, Bioproducts and Biorefining, 16(2), 480–493.
Valdés-García, A., Pérez-Rodríguez, M., & Díaz-García, M. E. (2024). Miniaturized capillary electrochromatography is used to determine glycerol purity in USP-grade samples. Microchemical Journal, 194, 108684.
Vasyliv, V., Mushtruk, M., Zheplinska, M., Mukoid, R., Tkachenko, S. (2022). Method of electrohydraulic activation of water-lime suspension in sugar production. In: Tonkonogyi, V., Ivanov, V., Trojanowska, J., Oborskyi, G., Pavlenko, I. (Eds.). Advanced manufacturing processes III. InterPartner 2021. Lecture notes in mechanical engineering. Springer, Cham.
Wang, Z., Liu, Y., Tang, J., & Zhang, H. (2024). Advanced glycerol purification technologies for pharmaceutical applications: Current status and prospects. Journal of Pharmaceutical Sciences, 113(2), 542–554.
Wang, Z., Liu, Y., Tang, J., & Zhang, H. (2024). Advanced glycerol quality control methods for pharmaceutical applications: Current status and prospects. Journal of Pharmaceutical Sciences, 113(2), 542–554.
Wilson, J. S., & Harris, P. L. (2019). Advanced purification strategies for glycerol from animal fats. Journal of Lipid Science and Technology, 121(3), 1800545.
Yang, F., Hanna, M. A., & Sun, R. (2012). Value-added uses for crude glycerol byproduct of biodiesel production. Biotechnology for Biofuels, 5(1), 13.
Yang, S., Wang, M., Chen, H., & Liu, Y. (2019). Challenges and limitations in quality control of biodiesel-derived glycerol. Renewable Energy, 143, 91–99.
Yateem, H., Afaneh, I., & Al-Rimawi, F. (2013). Optimum conditions for oleic acid esterification with ethanol. International Journal of Chemical Engineering and Applications, 4(6), 397–400.
Zhang, A., Yu, W., Hao, H., & Xie, Y. (2022). Engineered microorganisms for targeted glycerol production. Biotechnology Advances, 56, 107912.
Zhang, K., Wang, L., & Liu, Y. (2020). Economic viability of glycerol biorefinery: A comparative analysis of different valorization routes. Bioresource Technology, 307, 123223.
Zhang, W., Chen, X., & Li, Y. (2024). Selective zeolite catalysts for enhanced glycerol purification from biodiesel products. Microporous and Mesoporous Materials, 362, 112732.
Zheplinska, M., Mushtruk, M., & Salavor, O. (2021). Cavitational impact on electrical conductivity in the beet processing industry. In: Tonkonogyi, V., et al. (Eds.). Advanced manufacturing processes II. InterPartner 2020. Lecture notes in mechanical engineering. Springer, Cham.
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