Molecular genetic characterization of the beta-lactoglobulin gene of Bushuev dairy cattle, a local breed in Uzbekistan

B. S. Adilov; A. G. Sherimbetov; D. R. Ruzmetov; K. A. Giyasov; A. A. Nurmatov; Z. T. Mardonov; J. S. Shavkiev

doi:10.15421/0225018

B. S. Adilov Institute of Genetics and Plant Experimental Biology
A. G. Sherimbetov Institute of Genetics and Plant Experimental Biology
D. R. Ruzmetov Institute of Genetics and Plant Experimental Biology
K. A. Giyasov Institute of Livestock and Poultry Farming of the Republic of Uzbekistan
A. A. Nurmatov Institute of Livestock and Poultry Farming of the Republic of Uzbekistan
Z. T. Mardonov Institute of Livestock and Poultry Farming of the Republic of Uzbekistan
J. S. Shavkiev Institute of Genetics and Plant Experimental Biology

Keywords: local cattle breed, BLG gene, single nucleotide polymorphisms, SNP, phylogenetic analysis, evolution.

Abstract

The article presents the results of a molecular genetic study of the beta-lactoglobulin (BLG) of Bushuev dairy cattle , a local breed in Uzbekistan. Primers BLG_F1 and BLG_R1 were used to amplify the complete lacto-globulin gene of a B u shuev breeding bull. Then this BLG gene was sequenced. Blast analysis of obtained nucleotide sequence of this gene against NCBI GenBank database showed that it has the highest genetic similarity (99.98%) with the Wenshangaofeng breed of Chinese zebu cattle ( Bos indicus ). As a result of multiple alignment (ClustalW) of the BLG gene nucleotide sequence of the Bushuev breed with the Chinese zebu breed “Wenshangaofeng”, two novel SNP polymorphisms were identified: one 2711A>G, which is located in the 3rd intron and the second 3426G>C SNP polymorphism, which is located in the 4th i n tron. Phylogenetic analysis showed that the Bushuev breed is located in the Chinese cluster-1 that includes 5 Chinese zebu breeds: Wenshangaofeng, Weizhou, Guanling, Dabieshan and Jinjiang. Taking into consideration that the Bushuev dairy cattle breed was created by crossing local zebu cattle with Dutch and Swiss Brown bulls and some Simmentals and that the best crosses were bred inter se, it seems that the Bushuev breed retained the complete BLG gene from founder local zebu cattle, which possibly originated from the Chinese zebu Wenshangaofeng breed.

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