Antibiotic susceptibility of bacteria isolated from abscesses in cats
Abstract
The aim of this study was to identify opportunistic microorganisms in feline abscesses and determine their susceptibility to antibacterial drugs. Samples of biological material were cultured on appropriate nutrient media. Standard methods of microbiological practice were used to identify the isolated microorganisms. Pure cultures of microorganisms from abscesses were isolated on non-selective, selective, and differential nutrient media. Antibiotic susceptibility of the isolates was dete r mined by disk-diffusion method on the Mueller-Hinton agar. We studied 17 samples of biological material from feline a b scesses, from which 27 microorganisms were isolated: Enterobacteriaceae (8 isolates), Pseudomonas spp. (2 isolates), St a phylococcus spp. (7 isolates), Enterococcus spp. and Streptococcus pneumoniae (1 isolate each) and Candida auris (8 strains). One microorganism was isolated in 41.2% of cases, two pathogens in 52.9% of cases and three pathogens in 5.9% of cases. Multidrug resistance was determined in 73.7% of isolates. Only 26.3% of strains were susceptible to antibi o tics ( Staphylococcus aureus , S . epidermidis , Streptococcus pneumoniae , Enterococcus spp., Proteus spp.). Staphylococcus aureus was highly sensitive to nine out of ten antibiotics tested. The smallest were the zones of growth inhibition from the action of doxycycline and chloramphenicol against S. epidermidis . Isolates of gram-positive microorganisms were 100% susceptible to gentamicin and norfloxacin; to cefazolin 75%; to cefoperazone, kanamycin, amikacin 50%; to doxycycline, chloramphenicol and cefpirome 25%. Gram-negative Proteus spp. was resistant to norfloxacin. Regular antibiotic suscept i bility testing will allow us to study antibiotic susceptibility profiles of isolates in animal wound infections and create a system for monitoring the spread of antibiotic resistance. The results obtained can als o be used as a basis for developing a system for controlling and preventing the spread of antibiotic resistance.References
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