Molecular detection of Trichomonas vaginalis from women in Babylon province, Iraq
Abstract
Trichomonas vaginalis is one of the most important non-viral microorganisms that cause sexually transmitted infections with infection correlation with HIV that needs continuous surveillance. Our study was based on a collection of 100 vaginal swabs. These samples were tested using microscopy, measuring trophozoites, using Giemsa dye to diagnose T. vaginalis and also using PCR that targeted the 18S rRNA gene of the protozoan. The results revealed the presence of the parasite in 64 (64%) of the swabs taken as detected by the PCR. However, this parasite was found microscopically in only 7% of vaginal swab samples. In terms of geographical correlation, the findings demonstrated a significant connection between the prevalence rate and the sample collection regions, in which the highest rates of infection were in women from rural areas at 65.7% while in urban areas it was 60%. Regarding the age of the women infected, the highest prevalence 93.3% was found in the age group 26–35 years, compared to 50% in the 20–24 group and 53.3% in the older age group 36–40 years. The findings recorded higher prevalence rates (65% ) detected in women from rural districts compared to urban areas (60%).References
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