Effects of nutrient medium on various-age larvae of Hermetia illucens (Diptera, Stratiomyidae)
Abstract
The saprophage Hermetia illucens (Linnaeus, 1758) (Diptera, Stratiomyidae) plays a crucial role in the processing of organic wastes, thus contributing to the achievement of the global goals of sustainable development. However, its introduction in the European Union led to intense discussions about the expended application of this insect in new spheres. The article considers a laboratory culture of the fly H. illucens as an innovative nutritional product. The objective of the presented study was assessing the influence of nutrition medium on the dynamics of gains in the mass and biochemical composition of live larvae of the fly. In particular, we measured the mean gain in mass of the larvae over the period of consuming the diet, vitality of the larvae, and the content of protein, fat, ash, and water. In the studies, we tested seven diets for fly larvae in relation to the dynamics in their mass gain. The studies revealed that wheat bran and soybean meal led to the highest parameters of mass of the larvae at all development stages, with the highest gains measuring 10.15 and 10.76 g, respectively. Based on those results, the diet of larvae was further optimized: the main component, wheat bran, was supplemented with the following additional ingredients: soybean meal, yeasts, glucose, and vitamin В9. Such a composition of diet promoted gain in the mass of the larvae, which on average accounted for 14.06 g. The same changes were observed in the accumulation of proteins and fats – 16.8% and 20.4%, respectively, which may suggest heightened nutritional value of the larvae. Due to the high adaptability and varying abilities of biochemical composition, one can select an artificial medium in cases of different proportions. This study confirmed that larvae of the fly can be a source of protein and fatty acids for the food industry in the future and thus improve the qualitative and nutritional characteristics of end products.References
Araujo, J. P., Lorenzo, J. M., Cerqueira, J., Vazquez, J. A., Pires, P., Cantalapiedra, J., & Franco, D. (2015). Minhota breed cattle: Carcass characterisation and meat quality affected by sex and slaughter age. Animal Production Science, 56(12), 2086–2092.
Babytskiy, A. I., Bezsmertna, O. O., Moroz, M. S., Pavliuk, S. D., & Honcharenko, B. V. (2020). New records of Bradysia species (Diptera, Sciaridae) from Ukraine. Zoodiversity, 54(4), 329–340.
Bisconsin-Junior, A., Rodrigues, H., Behrens, J. H., da Silva, M. A. A. P., & Mariutti, L. R. B. (2022). “Food made with edible insects”: Exploring the social representation of entomophagy where it is unfamiliar. Appetite, 173, 106001.
Carpentier, J., Abenaim, L., Luttenschlager, H., Dessauvages, K., Liu, Y., Samoah, P., Francis, F., & Caparros Megido, R. (2024). Microorganism contribution to mass-reared edible insects: Opportunities and challenges. Insects, 15(8), 611.
Cullere, M., Tasoniero, G., Giaccone, V., Acuti, G., Marangon, A., & Dalle Zotte, A. (2018). Black soldier fly as dietary protein source for broiler quails: Meat proxymate composition, fatty acid and amino acid profile, oxidative status and sensory traits. Animal, 12(3), 640–647.
Cunha, C. F. D., Silva, M. B. D. O. D., & Cheung, T. L. (2023). Understanding the perception of edible insects. British Food Journal, 125(3), 980–993.
Deguara, A., Deguara, S., & Buhagiar, J. A. (2024). A multitrophic culture system for the production of black soldier fly larvae (Hermetia illucens). Discover Food, 4(1), 56.
Dickerson, A. J., Lemke, N. B., Li, C., & Tomberlin, J. K. (2024). Impact of age on the reproductive output of Hermetia illucens (Diptera: Stratiomyidae). Journal of Economic Entomology, 117(4), 1225–1234.
Elhag, O., Zhou, D., Song, Q., Soomro, A.A., Cai, M., Zheng, L., Yu, Z., & Zhang J.(2017). Screening, expression, purification and functional characterization of novel antimicrobial peptide genes from Hermetia illucens (L.). PloS One, 12(1), e0169582.
Fahmy, L., Generalovic, T., Ali, Y. M., Seilly, D., Sivanesan, K., Kalmar, L., Pipan, M., Christie, G., & Grant, A. J. (2024). A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties. BMC Microbiology, 24(1), 167.
Gahukar, R. T. (2011). Entomophagy and human food security. International Journal of Tropical Insect Science, 31(3), 129–144.
He, Y., Peng, H., Jin, M., Wang, J., Li, S., Li, M., Zhu, T., Zhang, L., Chen, X., & Zhou, Q. (2024). Application evaluation of black soldier fly (Hermetia illucens) larvae oil in shrimp feed: Effects on growth performance, antioxidant capacity and lipid metabolism. Aquaculture Reports, 36, 102174.
Knowles, T., Moody, R., & McEachern, M. G. (2007). European food scares and their impact on EU food policy. British Food Journal, 109(1), 43–67.
Koyunoglu, C. (2024). Biofuel production utilizing black soldier fly (Hermetia illucens): A sustainable approach for organic waste management. International Journal of Thermofluids, 23, 100754.
Laurenza, E. C., & Carreño, I. (2015). Edible insects and insect-based products in the EU: Safety assessments, legal loopholes and business opportunities. European Journal of Risk Regulation, 6(2), 288–292.
Liu, T., Awasthi, M. K., Awasthi, S. K., Duan, Y., & Zhang, Z. (2020). Effects of black soldier fly larvae (Diptera: Stratiomyidae) on food waste and sewage sludge composting. Journal of Environmental Management, 256, 109967.
Lo, D., Loho, L., Afandi, F. A., Romulo, A., Tedjakusuma, F., Soma, T., Kaburuan, E. R., Zulkarnain, A., & Khatri-Chhetri, R. (2024). Effect of different feed on nutritional content of black soldier fly (Hermetia illucens): A systematic review and meta-analysis. Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 7(1), 66–78.
Lu, S., Taethaisong, N., Meethip, W., Surakhunthod, J., Sinpru, B., Sroichak, T., Archa, P., Thongpea, S., Paengkoum, S., Aprilia, R., Purba, P., & Paengkoum, P. (2022). Nutritional composition of black soldier fly larvae (Hermetia illucens L.) and its potential uses as alternative protein sources in animal diets: A review. Insects, 13(9), 831.
Makkar, H. P., Tran, G., Heuze, V., & Ankers, P. (2014). State of the art on use of insects as animal feed. Animal Feed Science and Technology, 197, 1–33.
Megido, R. C., Sablon, L., Geuens, M., Brostaux, Y., Alabi, T., Blecker, C., Drugmand, D., Haubruge, E., & Francis, F. (2014). Edible insects acceptance by Belgian consumers: Promising attitude for entomophagy development. Journal of Sensory Studies, 29(1), 14–20.
Meyer-Rochow, V. B., & Chakravorty, J. (2013). Notes on entomophagy and entomotherapy generally and information on the situation in India in particular. Applied Entomology and Zoology, 48, 105–112.
Morales-Ramos, J. A., Tomberlin, J. K., Miranda, C., & Rojas, M. G. (2024). Rearing methods of four insect species intended as feed, food, and food ingredients: A review. Journal of Economic Entomology, 117(4), 1210–1224.
Nadeau, L., Nadeau, I., Franklin, F., & Dunkel, F. (2015). The potential for entomophagy to address undernutrition. Ecology of Food and Nutrition, 54(3), 200–208.
Peshuk, L. V., Gorbach, A. Y., & Bakhmach, V. A. (2017). Prospects for the use of plant and animal proteins in the technology of meat products. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 19(80), 68–73.
Rodriguez-Rodríguez, M., Sánchez-Muros, M. J., Vargas-García, M. D. C., Varga, Á. T., Fabrikov, D., & Barroso, F. G. (2024). The effects of slaughter methods and drying temperatures on the protein hydrolysis of black soldier fly larvae meal. Animals, 14(11), 1709.
Salomone, R., Saija, G., Mondello, G., Giannetto, A., Fasulo, S., & Savastano, D. (2017). Environmental impact of food waste bioconversion by insects: Application of life cycle assessment to process using Hermetia illucens. Journal of Cleaner Production, 140, 890–905.
Scala, A., Cammack, J. A., Salvia, R., Scieuzo, C., Franco, A., Bufo, S. A., Tomberlin, J. K., & Falabella, P. (2020). Rearing substrate impacts growth and macronutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae produced at an industrial scale. Scientific Reports, 10(1), 19448.
Shelomi, M. (2016). The meat of affliction: Insects and the future of food as seen in Expo 2015. Trends in Food Science and Technology, 56, 175–179.
Sheppard, D. C., Newton, G. L., Thompson, S. A., & Savage, S. (1994). A value added manure management system using the black soldier fly. Bioresource Technology, 50(3), 275–279.
Siegrist, M. (2008). Factors influencing public acceptance of innovative food technologies and products. Trends in Food Science and Technology, 19(11), 603–608.
Slobodianiuk, N. M., Holembovska, N. V., Menchynska, A. A., Androshchuk, O. S., & Tulub, D. O. (2018). Tekhnolohiia pererobky ryby [Fish processing technology]. Comprint, Kyiv (in Ukrainian).
Smetana, S., Mathys, A., Knoch, A., & Heinz, V. (2015). Meat alternatives: life cycle assessment of most known meat substitutes. The International Journal of Life Cycle Assessment, 20, 1254–1267.
Smetana, S., Palanisamy, M., Mathys, A., & Heinz, V. (2016). Sustainability of insect use for feed and food: Life cycle assessment perspective. Journal of Cleaner Production, 137, 741–751.
Spranghers, T., Noyez, A., Schildermans, K., & De Clercq, P. (2017). Cold hardiness of the black soldier fly (Diptera: Stratiomyidae). Journal of Economic Entomology, 110(4), 1501–1507.
Statkevych, O., & Drozda, V. (2020). Eco-geographical components of natural population variability of ectoparasites Habrobracon hebetor (Say, 1836) (Hymenoptera, Braconidae). Türkiye Tarımsal Araştırmalar Dergisi, 7(3), 280–286.
Tognocchi, M., Abenaim, L., Adamaki-Sotiraki, C., Athanassiou, G. C., Rumbos, I. C., Mele, M., Conti, B., & Conte, G. (2024). Effect of different diet composition on the fat profile of two different black soldier fly larvae populations. Animal, 18, 101205.
Tomberlin, J. K., Van Huis, A., Benbow, M. E., Jordan, H., Astuti, D. A., Azzollini, D., Banks, I., Bava, V., Borgemeister, C., & Cammack, J. A. (2015). Protecting the environment through insect farming as a means to produce protein for use as livestock, poultry, and aquaculture feed. Journal of Insects as Food and Feed, 1(4), 307–309.
Van Huis, A., Oonincx, D. G., Rojo, S., & Tomberlin, J. K. (2020). Insects as feed: House fly or black soldier fly?. Journal of Insects as Food and Feed, 6(3), 221–229.
Van Zanten, H. H., Van Ittersum, M. K., & De Boer, I. J. (2019). The role of farm animals in a circular food system. Global Food Security, 21, 18–22.
Veldkamp, T., & Bosch, G. (2015). Insects: A protein-rich feed ingredient in pig and poultry diets. Animal Frontiers, 5(2), 45–50.
Vos, E. (2000). EU food safety regulation in the aftermath of the BSE crisis. Journal of consumer Policy, 23(3), 227–255.
Wang, Y. S., & Shelomi, M. (2017). Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods, 6(10), 91.
Сiсkovа, H., Newton, G. L., Lacy, R. C., & Kozаnek, M. (2015). The use of fly larvae for organic waste treatment. Waste Management, 35, 68–80.
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