Influence of solution of lactoprotein with sorbitol on ultrastructural changes in lungs of rats in the condition of burn shock
Abstract
This article gives a theoretical substantiation and a new experimental solution of a scientific problem aimed at increasing the effectiveness of pharmacotherapy on the morphofunctional state of the lungs of rats under conditions of burn shock by using a combined colloid-hyperosmolar infusion solution – lacto-protein with sorbitol. The administration of the test solution at a dose of 10 ml/kg for 7 days in rats with modelled burn shock reduced ultrastructural changes in the lungs triggered by burn shock. It has been proved that in the conditions of shock, colloid-hyperosmolar infusion lacto-protein with sorbitol solution facilitates the restoration of vascular endothelium and fluid retention in the microcirculatory channel and improves the morphofunctional state of the aerohematic barrier of the lungs, stimulates the activity of the alveolar macrophages and the secretory function of the type II alveolocytes producing surfactant. At day 7 of burn shock, when 0.9% of NaCl was injected, significant changes were observed in the respiratory unit: part of the alveoli had considerably enhanced clearance of blood capillaries, which had platelets, neutrophils and altered forms of erythrocytes. At day 7 of burn shock in the lungs of the rats given an infusion of colloid-hyperosmolar solution – lactoprotein with sorbitol, the ultrastructure of the components of the lung cells had improved in comparison with 3 days. Luminosity of the hemocapillary parts was moderate, mainly with erythrocytes. The walls of endothelial cells had elongated nuclei with invaginations of nuclear membranes and clear contours. Their cytoplasmic regions were not widespread, with moderate electron densities. In type II alveolocytes, during this experiment, a lower degree of damage to the nucleus and organelles in the cytoplasm was established, and there were signs of a renewal of the secretory function of these cells. In the cytoplasm, hypertrophied mitochondria with clear crystals, different sizes of secretory granules, which had a different density, indicating their formation, were observed. According to the magnitude of the cytoprotective effect on lung cells under conditions of burn shock, the lactoprotein with sorbitol solution was shown to be superior in comparison with the physical solution (0.9% NaCl). The study of functional, biochemical and molecular genetic parameters that characterize the state of the aerohematic barrier under the conditions of using lactoprotein with sorbitol solution in the case of burn injuries of the skin will allow researchers to comprehensively evaluate the mechanisms of the pulmonary protective effect of this preparation and to experimentally substantiate the expediency of its use in clinical practice for pharmaco-correction of burn shock.References
Cherkasov, V. G., Kovalchuk, А. I., Dzevulskaya, I. V., & Cherkasov, E. V. (2015). Evaluation of the effect of infusion of composite hyperosmolar solutions on the structure of the neuroimmunoendocrine system organs in burn disease. European International Journal of Science and Technology, 4(9), 51–61.
Directive 2010/63/EC of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (2010). Official Journal of the European Union, 276, 33–79.
Guminskiy, Y. I., Gunas, I. V., Ocheretna, N. P., & Bashinska, O. I. (2017). Micromorphometric changes in rats’ spleen in the first 7 days after skin burns and under application of infusion solutions. Reports of Morphology, 23(2), 240–244.
Jacob, S., Zhu, Y., Kraft, R., Cotto, C., Carmical, J. R., Wood, T. G., & Cox, R. A. (2015). Physiologic and molecular changes in the tracheal epithelium of rats following burn injury. International Journal of Burns and Trauma, 5(1), 36–45.
Kaddoura, I., Abu-Sittah, G., Ibrahim, A., Karamanoukian, R., & Papazian, N. (2017). Burn injury: Review of pathophysiology and therapeutic modalities in major burns. Annals of Burns and Fire Disasters, 30(2), 95–102.
Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. The Journal of Trauma, 32(5), 557–563.
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