Correlation between aerobic and anaerobic microflora of the urogenital tract at dysbiosis in women of Dnipropetrovsk city

  • A. H. Yaderna Diagnostic Center of Dnipropetrovsk Medical Academy
  • L. P. Golodok Oles Honchar Dnipropetrovsk National University
  • A. I. Vinnikov Oles Honchar Dnipropetrovsk National University
Keywords: PCR in real time, urogenital tract, child-bearing potential

Abstract

The method of polymerase chain reaction (PCR) in real-time was used to analyze the quantitative characteristics of normal and potentially pathogenic aerobic/facultative-anaerobic and anaerobic biota in the urethra, cervical channel and vagina in healthy women aged 10–40. The biota of all the women under 40 years and some of women older than 40 was mostly represented by lactobacilli. Microbialcomposition of the biocenosis in some women older than 40 is characterized by reduction in quantity of lactobacilli and their replacement by anaerobic microorganisms, mainly, such as Atopobium vaginae (16%), Gardnerella vaginalis (12%), Megasphaera spp. (8%), Dialister spp. (8%), Eubacterium spp. (8%) and Porphyromonas spp. (4%). Rarely, a wide range of other pathogens plays its role, including inter alia: Trichomonas vaginalis (8%), Mycoplasma genitalium (4%), Neisseria gonorrhoeae (2%) andChlamydia trachomatis (1%). The most frequent are the following strain associations: A. vaginae andG. vaginalis,Eubacterium spp. andPorphyromonas spp.,U. (urealyticum + parvum) andM. genitalium,N. gonorrhoeae andCh. trachomatis,U. (urealyticum + parvum) andCandida spp. This is connected with anatomical and physiological characteristics of genitals, hormonal and immune system action. Quantitative study of the biota of urogenital tract in Dnipropetrovsk women with the use of real-time PCR is the sensitive method for diagnosing both physiological and pathological changes, and dysbiotic disorders at early stages and preventing their further development into more serious forms.

References

Aila, N.A, Tency, L., Claeys, G., Verstraelen, H., Saerens, B., 2010. Comparison of different sampling techniques and of different culture methods for detection of group B Streptococcus carriage in pregnant women. BMC Infect. Dis. 10, 167–285.

Baiulescu, M., Hannon, P.R., Marcinak, J.F., Janda, W.M., Schreckenberger, P.C., 2002. Chronic vulvovaginitis caused by antibiotic-resistant Shigella flexneri in a prepubertal child. Pediatr. Infect. Dis. 21(2), 170–172. >>doi: 10.1097/00006454-200202000-00019

Boldyrev, M.N., Lypova, E.V., Trofimov, D.Y., Vitvitskaya, Y.G., Gus'kova, I.A., 2010. Osobennosti bioty urogenital'nogo trakta zdorovyh zhenshhin reproduktivnogo vozrasta v issledovanii metodom PCR v rezhime real'nogo vremeni [Features of biota of the urogenital tract of healthy women of reproductive age in the study by PCR real time]. J. Dermatol. Venereol. 1, 80–84 (in Russian).

Cauci, S., Guashino, S., De Aloysio, D., Driussi, S., De Santo, D., 2002. Prevalence of bacterial vaginosis and vaginal flora changes in peri- and postmenopausal women. Clin. Microbiol. 40(6), 2147–2152. >>doi: 10.1128/JCM.40.6.2147-2152.2002

Cox, R.A., Slack, M.P., 2002. Clinical and microbiological features of Haemophilus influenzae vulvovaginitis in young girls. J. Clin. Pathol. 55, 961–964. >>doi: 10.1136/jcp.55.12.961

David, N., Fredricks, M.D., Tina, L., Fiedler, B.S., Jeanne, M., Marrazzo, M.D., 2005. Molecular identification of bacteria associated with bacterial vaginosis. N. Engl. J. Med. 353(18), 1899–1911.

Donder, G.G., Vereecken, A., Bosmans, E., Dekeersmaecker, A., Salembier, G., Spitz, B., 2002. Definition of a type of abnormal vaginal flora that is distinct from bacterial vaginosis: Aerobic vaginitis. BJOG 109(1), 34–43. >>doi: 10.1111/j.1471-0528.2002.00432.x

Falagas, M.E., Betsi, G.I., Athanasiou, S., 2007. Probiotics for the treatment of women with bacterial vaginosis. Clin. Microbiol. Infect. 13(7), 657–664. >>doi 10.1111/j.1469-0691.2007.01688.x

Ferris, M.J., Masztal, A., Aldridge, K.E., Fortenberry, J.D., Fidel, P.L., Jr, Martin, D.H., 2004. Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis. BMC Infect. Dis. 4(5), 65–70. >>doi: 10.1186/1471-2334-4-5

Fischer, G.O., 2001. Vulval disease in pre-pubertal girls. J. Dermatol. 42(4), 225–234.

Fredricks, D.N., Fiedler, T.L., Thomas, K.K., Oakley, B.B., Marrazzo, J.M., 2007. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J. Clin. Microbiol. 45(10), 3270–3276. >>doi: 10.1128/JCM.01272-07

Gupta, C., Briski, J., 2004. Comparison of two culture media and three sampling techniques for sensitive and rapid screening of vaginal colonization by group B Streptococcus in pregnant women. J. Clin. Microbiol. 42(9), 3975–3977. >>doi: 10.1128/JCM.42.9.3975-3977.2004

Кaites, K.B., Katz, B., Schelonka, R.L., 2005. Mycoplasmas and Ureaplasmas as neonatal pathogens. Clin. Microbiol. Rev. 18(4), 757–789.

Klebanoff, M.A., Schwebke, J.R., Zhang, J., Nansel, T.R., Yu, K.F., Andrews, W.W., 2004. Vulvovaginal symptoms in women with bacterial vaginosis. Obstet. Gynecol. 104(2), 267–272. >>doi: 10.1097/01.AOG.0000134783.98382.b0

Koumfns, E.H., Markowitz, L.E., Hogan, V., 2002. Bacterial vaginosis. Hogan. Clin. Infect. Dis. 15(2), 152–172.

Larsen, B., Monif, G.R., 2001. Understending the bacterial flora of the female genital tract. Clin. Infect. Dis. 32, 69–77. >>doi: 10.1086/318710

Melis, G.B., Ibba, M.T., Steri, B., 2000. Role of pH as a regulator of vaginal physiological environment. Minerva. Ginecol. 52(4), 111–121.

Newton, E.R., Piper, J.M., Shain, R.N., Perdue, S.T., Peairs, W., 2001. Predictors of the vaginal microflora. J. Obstet. Gynecol. 184(5), 845–853. >>doi: 10.1067/mob.2001.113848

Reid, G., Bocking, A., 2003. The potential for probiotics to prevent bacterial vaginosis and preterm labor. J. Obstet. Gynecol. 189, 1202–1208. >>doi: 10.1067/S0002-9378(03)00495-2

Reid, G., Dols, J., Miller, W., 2009. Targeting the vaginal microbiota with probiotics as a means to counteract infections. Curr. Opin. Clin. Nutr. Met. Care 12, 583–587. >>doi: 10.1097/MCO.0b013e328331b611

St John, E., Mares, D.A., Spear, G.T., 2007. Bacterial vaginosis and host immunity. Curr. HIV/AIDS Rep. 4 (1), 22–28. >>doi: 10.1007/s11904-007-0004-y

Witkin, S.S., Linhares, I.M., Giraldo, P., Ledger, W.J., 2007. An altered immunity hypothesis for the development of symptomatic bacterial vaginosis. Clin. Infect. Dis. 44(4), 554–557. >>doi: 10.1086/511045

Published
2014-07-12
How to Cite
Yaderna, A. H., Golodok, L. P., & Vinnikov, A. I. (2014). Correlation between aerobic and anaerobic microflora of the urogenital tract at dysbiosis in women of Dnipropetrovsk city. Regulatory Mechanisms in Biosystems, 5(2), 110-114. https://doi.org/10.15421/021421