Antagonistic activity of Bacillus probiotics against bacteria isolates of oral cavity of patients with periodontitis

  • O. Rivis Uzhgorod National Unisversity
  • M. Kryvtsova Uzhgorod National Unisversity
  • V. Nikolajchuk Uzhgorod National Unisversity
Keywords: microflora, periodontitis, Bacillus probiotics, microbial antagonism

Abstract

It is admitted that the normal human microflora plays an important role in supporting homeostasis, forming immune mechanisms and metabolism. Nowadays, there is a constant growth of different diseases due to microbiological imbalance in a human organism. Preparations containing “good bacteria” have been used for therapeutic purposes since ancient times. The mechanism of probiotics influence comprises their ability to compete for adhesion sites with the pathogens, to exhibit antagonistic activity and stimulate the immune system of a host. Most of probiotics commonly used are the spores of Bacillus. Initially the main focus of their use was the prevention of gastrointestinal disorders. So, the use of probiotics in dental practice is a poorly studied area. In recent years, probiotics have been investigated to provide the oral health. Therefore the study of using probiotics for correction of the oral microflora in people with inflammatory diseases of the periodontal tissues is promising. Our previous studies have shown changes in microbial community of an oral cavity in patients with periodontitis. In particular, the reducing number of obligate microorganisms and increasing number of pathogens was demonstrated. The paper describes the current data on the potential benefits and basic properties of the Bacillus spore probiotics, which demonstrate the viability and relevance in dental practice. The study tested antagonistic activity of commercial Bacillus probiotics "Biosporin" ("Biopharma", Ukraine), "Subalinum" ("Biopharma", Ukraine), "Normaflore" ("Sanofi-Aventis Zrt.", Hungary) and "Enterogermina" ("Sanofi-Synthelabo SpA", Italy) against clinical strains of microorganisms isolated from the oral cavity of patients with periodontitis. Thus, further studies on the role of spore probiotics in correction of the oral cavity microflora as a part of complex treatment of periodontitis should be carried out.

References

Agarwal, E., Bajaj, P., Guruprasad, C.N., Naik, S., Pradeep, A.R., 2011. Probiotics: A novel step towards oral health. Archives of Oral Sciences & Research 1(2), 108–115.

Casula, G., Cutting, S.M., 2002. Bacillus probiotics: Spore germination in the gastrointestinal tract. Applied and Environmental Microbiology 68(5), 2344–2352. >>doi: 10.1128/AEM.68.5.2344-2352.2002

Duc, L.H., Hong, H.A., Barbosa, T.M., Henriques, A.O., Cutting, S.M., 2004. Characterization of Bacillus probiotics available for human use. Applied and Environmental Microbiology 70(4), 2161–2171. >>doi: 10.1128/AEM.70.4.2161-2171.2004

Jain, P., Sharma, P., 2012. Probiotics and their efficacy in improving oral health: A review. Journal of Applied Pharmaceutical Science 2(11), 151–163.

Hong, H.A., Duc, L.H., Cutting, S.M., 2005. The use of bacterial spore formes as probiotics. FEMS Microbiologe Reviews 29, 813–835. >>doi: 10.1016/j.femsre.2004.12.001

Marsh, P.D., 2000. Role of the oral microflora in health. Microbial Ecology in Health and Diseases 12, 130–137. >>doi: 10.1080/089106000750051800

>>doi: 10.3402/mehd.v12i3.8195

Meurman, J.H., 2005. Probiotics: Do they have a role in oral medicine and dentistry? Eur. J. Oral. Sci. 113, 188–196. >>doi: 10.1111/j.1600-0722.2005.00191.x

Mohanty, R., Nazareth, B., Shrivastava, N., 2012. The potential role of probiotics in periodontal health. RSBO 9(1), 85–88.

Narang, S., Gupta, R., Narang, A., 2011. Probiotics in oral healthcare – A review. International Journal of Scientific & Engineering Research 2(1), 1–5.

Reddy, R.S, Swapna, L.A., Ramesh, T., Rajesh Singh, T., Vijayalaxmi, N., Lavanya, R., 2011. Bacteria in oral health – probiotics and prebiotics. A review. International Journal of Biological & Medical Research 2(4), 1226–1233.

Rivis, O.J., Kryvcova, M.V., Nikolajchuk, V.I., Semenova, G.M., Barani, J.A., 2012. The microflora of the oral cavity of the people with inflammatory diseases of periodontal tissue in Uzhgorod area [Mikroflora rotovoi' porozhnyny ljudej z zapal'nymy zahvorjuvannjamy tkanyny parodontu v Uzhgorods'komu rajoni]. Visnyk Problem Biologii' i Medycyny 3(1), 121–124 (in Ukrainian).

Rusyn, V.V., Potapchuk, A.M., Petrov, V.O., Belej, O.L., Bojko, N.V., 2012. Prospects of the bacilli based biopreparations implementation in the dental practice [Perspektyvy vykorystannja bacyljarnyh biopreparativ u stomatologijanij praktyci]. Biomedical and Biosocial Anthropology 15, 134–139 (in Ukrainian).

Ryzhkova, T.A., Babych, J.M., Kalinichenko, S.V., Skljar, N.I., Shkodovs'ka, N.J., Dubova, L.M., Danylina, S.S., Matjunina, D.M., 2009. Manifestation of antagonistic properties of bacteria under aerobic and microaerophilic growth conditions [Projav antagonistychnyh vlastyvostej mikroorganizmiv pry aerobnyh i mikroaerofil'nyh umovah i'h kul'tyvuvannja]. Eksperymental'na i Klinichna Medycyna 1, 47–52 (in Ukrainian).

Simonova, E.V., Ponomareva, O.A., 2008. The role of normal microflora in supporting human health [Rol' normal'noj mikroflory v podderzhanii zdorov'ja cheloveka]. Sibirskij Medicinskij Zhurnal 8, 20–25 (in Russian).

Skrypnik, I.N., Maslova, A.S., 2009. Modern sporecreating probiotics in clinical practice [Sovremennye sporoobrazujushhie probiotiki v klinicheskoj parktike]. Suchasna Gastroenterologіja 47(3), 81–90 (in Russian).

Stamatova, I.V., 2010. Probiotics activity of Lactobacillus delbrueckii subsp. bulgaricus in the oral cavity: Academic dissertation. Helsinki, 82 p.

Twetman, S., Stecksen-Blicks, C., 2008. Probiotics and oral health effects in children. Int. J. of Pead. Dent. 18, 3–10.

Vishnu, H.P., 2012. Probiotics and oral health. Oral health care – pediatric, research, epidemiology and clinical practices 11, 195–204.

Published
2013-03-17
How to Cite
Rivis, O., Kryvtsova, M., & Nikolajchuk, V. (2013). Antagonistic activity of Bacillus probiotics against bacteria isolates of oral cavity of patients with periodontitis. Regulatory Mechanisms in Biosystems, 4(1), 10-13. https://doi.org/10.15421/021302